Fish sperm cryopreservation is a well-established technique allowing for artificial insemination on a commercial scale. The extent of proteome alterations in seminal plasma and sperm due to cryopreservation, however, is not known. This study was conducted to evaluate the effect of cryopreservation on motility variables of sterlet Acipenser ruthenus sperm and to detect the differences in protein profiles of fresh and cryopreserved sterlet sperm and seminal plasma. Fresh sperm had 89 ± 3% motility and 160 ± 14 μm/s curvilinear velocity at 15 s post-activation. The motility rate of cryopreserved sperm (37 ± 5%) was less at 15 s post-activation. No difference (ANOVA; P > 0.05) in mean curvilinear velocity of fresh and cryopreserved sperm was detected. The protein profiles of seminal plasma and sperm were characterized using comparative proteomics to determine the influence of cryopreservation. Six altered protein spots in seminal plasma and thirteen altered spots in sperm were detected in fresh and thawed sperm. Subsequent protein characterization suggested that the proteins identified were involved in sperm metabolism, cytoskeleton, and stress response. The results broaden the understanding of the effects of cryopreservation and identify the proteins associated with cryo-injury. These data may help to determine the function of altered proteins and provide new insights into improving sperm cryopreservation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.anireprosci.2018.03.025 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enzymatic Antioxidants and Their Differential Response to Cryopreservation in Japanese Quail Semen.
Reprod Domest Anim
January 2025
Department of Zoology, Wildlife and Fisheries, Pir Mehr Ali Shah Arid Agriculture University of Rawalpindi, Rawalpindi, Pakistan.
A triad of enzymatic antioxidants viz., catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) constitutes a first line of defence against any redox imbalances in the semen. Cryopreservation enabling long term storage of semen also prompts generation of surplus reactive oxygen species (ROS) in the cells with waned antioxidants, hampering the full exploitation of this process.
View Article and Find Full Text PDFTrichomoniasis in Men: A Neglected Factor in Male Infertility?
Acta Parasitol
January 2025
Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, P.O. Box: 65157838736, Hamadan, Iran.
Purpose: This study is aimed to detect the frequency of trichomoniasis, a sexually transmitted infection caused by an anaerobic protozoan Trichomonas vaginalis, in men referred to the Fertility and Infertility Research Center Hamadan University of Medical Sciences.
Methods: In this cross-sectional study, a group of 197 male volunteers who sought medical attention for issues related to infertility participated. The urine and semen samples were collected in sterile conditions.
Toxoplasma gondii Infection in the Male Reproductive System: A Systematic Review.
Acta Parasitol
January 2025
Reproduction Group, Department of Microbiology and Parasitology, School of Medicine, University of Antioquia- UdeA, Medellín, Antioquia, Colombia.
Purpose: Toxoplasmosis is a worldwide widespread parasitic infection; it affects about 30% of the global population, either through acute toxoplasmosis or its sequels. Even though the male reproductive system is not the primary target for Toxoplasma gondii (T. gondii), studies have inquired into the possibility of presenting repercussions in this system directly or indirectly due to toxoplasmosis.
View Article and Find Full Text PDFPhosphodiesterase Type 5 Inhibitors in Male Reproduction: Molecular Mechanisms and Clinical Implications for Fertility Management.
Cells
January 2025
Third Department of Urology, Attikon University Hospital, School of Medicine, National and Kapodistrian University of Athens, 12462 Athens, Greece.
Phosphodiesterases, particularly the type 5 isoform (PDE5), have gained recognition as pivotal regulators of male reproductive physiology, exerting significant influence on testicular function, sperm maturation, and overall fertility potential. Over the past several decades, investigations have expanded beyond the original therapeutic intent of PDE5 inhibitors for erectile dysfunction, exploring their broader reproductive implications. This narrative review integrates current evidence from in vitro studies, animal models, and clinical research to clarify the roles of PDEs in effecting the male reproductive tract, with an emphasis on the mechanistic pathways underlying cyclic nucleotide signaling, the cellular specificity of PDE isoform expression, and the effects of PDE5 inhibitors on Leydig and Sertoli cell functions.
View Article and Find Full Text PDFPosterior Urethral Valves and Fertility: Insight on Paternity Rates and Seminal Parameters.
Diseases
January 2025
Department of Urology, Magna Graecia University of Catanzaro, Viale Europa, 88100 Catanzaro, Italy.
Background: Posterior urethral valves (PUVs) represent the most common cause of male congenital lower urinary tract obstruction, often responsible for renal dysplasia and chronic renal failure. Despite recent improvements in patients' outcomes thanks to prenatal ultrasound early diagnosis, PUVs can still impact sexual function and fertility. This study aims to review the available evidence on fertility in PUV patients, examining paternity rates and semen parameters.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!