AI Article Synopsis
- Cell surface receptors are crucial for cell communication and can indicate diseases or be targeted for therapies.
- A new method called the split proximity circuit (SPC) allows for the autonomous detection of receptor clusters using antibodies and DNA hybridization for signal amplification.
- This method effectively visualizes the human epidermal growth factor receptor (HER) family in different cell types, using simple imaging techniques without needing extra washing steps.
Article Abstract
Cell surface receptors play an important role in mediating cell communication and are used as disease biomarkers and therapeutic targets. We present a one-pot molecular toolbox, which we term the split proximity circuit (SPC), for the autonomous detection and visualization of cell surface receptor clusters. Detection was powered by antibody recognition and a series of autonomous DNA hybridization to achieve localized, enzyme-free signal amplification. The system under study was the human epidermal growth factor receptor (HER) family, that is, HER2:HER2 homodimer and HER2:HER3 heterodimer, both in cell lysate and in situ on fixed whole cells. The detection and imaging of receptors were carried out using standard microplate scans and confocal microscopy, respectively. The circuit operated specifically with minimal leakages and successfully captured the receptor expression profiles on three cell types without any intermediate washing steps.
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| http://dx.doi.org/10.1021/acs.analchem.8b00722 | DOI Listing |
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