Aims: A new multiplex qPCR, targeting Mycoplasma (M.) hyopneumoniae, M. hyorhinis and M. flocculare, was developed and the relationship between detection of those mycoplasma species and the extent of gross pneumonia-like lesions in slaughtered pigs lungs were investigated.
Methods And Results: The multiplex qPCR method targets the p102, p37 and fruA genes and has detection limits of 14, 146 and 16 genome equivalents μl for M. hyopneumoniae, M. hyorhinis and M. flocculare, respectively. In all, 671 lungs were collected and analysed, among them 666 were scored for macroscopic pneumonia and categorized according to the extent of the lesions (no or minor lesions, moderate lesions and extensive lesions). According to results of multiplex qPCR, 59·5% were positive for M. hyopneumoniae, 3·4% for M. hyorhinis and 34·7% for M. flocculare, with on average, 3·1 × 10 , 9·7 × 10 and 5·7 × 10 genome equivalents of mycoplasma ml , respectively. More results showed that no or minor lesions were associated with multiplex qPCR-negative results or qPCR-positive results for M. flocculare. Moderate to extensive lesions were positively correlated with qPCR-positive results for M. hyopneumoniae. Extensive lesions were associated with qPCR-positive results for at least two mycoplasma species (M. hyopneumoniae and M. hyorhinis).
Conclusion: The findings also indicated that M. hyopneumoniae and M. hyorhinis significantly increased the odds for a lung to have macroscopic pneumonia. No relationship was found between the extent of lesions and the mycoplasma genome load.
Significance And Impact Of The Study: This new multiplex qPCR appears to be specific, sufficiently sensitive and repeatable. The validation of this method with field samples guarantees its use for field epidemiological investigations, particularly to gain more insight into the aetiology of the porcine respiratory disease complex.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/jam.13770 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative assessment of the performance of a commercial fluorescent microsphere immunoassay and three commercial ELISAs for Mycoplasma hyopneumoniae serum antibody detection.
Vet Immunol Immunopathol
October 2024
Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, United States. Electronic address:
Mycoplasma hyopneumoniae (M. hyopneumoniae) is a significant porcine respiratory disease complex pathogen, prompting many swine farms and production systems to pursue M. hyopneumoniae elimination strategies.
View Article and Find Full Text PDFFunctional surface expression of immunoglobulin cleavage systems in a candidate Mycoplasma vaccine chassis.
Commun Biol
June 2024
Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3001, Bern, Switzerland.
Article Synopsis
- The Mycoplasma Immunoglobulin Binding/Protease (MIB-MIP) system is a virulence factor found in pathogenic Mollicutes, like Mycoplasma feriruminatoris, that helps these bacteria evade the immune system by cleaving host immunoglobulins.
- Research using -omics technologies showed that M. feriruminatoris has four MIB-MIP gene copies with varying expression levels, all transcribed as operons through different promoters.
- Although MIB-MIP proteins can be functionally expressed on the surface of M. feriruminatoris, introducing MIB-MIP systems from other Mollicutes like Mesomycoplasma hyorhinis was unsuccessful, but
Design of a New Vaccine Prototype against Porcine Circovirus Type 2 (PCV2), and Based on Multiple Antigens Microencapsulation with Sulfated Chitosan.
Vaccines (Basel)
May 2024
Centro Biotecnológico Veterinario, Biovetec, Departamento de Ciencias Biológicas, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa 11735, La Pintana, Santiago 8320000, Chile.
This work evaluated in vivo an experimental-multivalent-vaccine (EMV) based on three Porcine Respiratory Complex (PRC)-associated antigens: Porcine Circovirus Type 2 (PCV2), (Mhyop) and (Mhyor), microencapsulated with sulfated chitosan (M- ChS + PRC-antigens), postulating chitosan sulphate (ChS) as a mimetic of the heparan sulfate receptor used by these pathogens for cell invasion. The EMV was evaluated physicochemically by SEM (Scanning-Electron-Microscopy), EDS (Energy-Dispersive-Spectroscopy), Pdi (Polydispersity-Index) and zeta potential. Twenty weaned pigs, distributed in four groups, were evaluated for 12 weeks.
View Article and Find Full Text PDFRetrospective Analysis of the Detection of Pathogens Associated with the Porcine Respiratory Disease Complex in Routine Diagnostic Samples from Austrian Swine Stocks.
Vet Sci
October 2023
Department for Farm Animals and Veterinary Public Health, University Clinic for Swine, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.
The diagnostic workup of respiratory disease in pigs is complex due to coinfections and non-infectious causes. The detection of pathogens associated with respiratory disease is a pivotal part of the diagnostic workup for respiratory disease. We aimed to report how frequently certain viruses and bacteria were detected in samples from pigs with respiratory symptoms in the course of routine diagnostic procedures.
View Article and Find Full Text PDFInteractions of and/or with Serotype 2 Using In Vitro Co-Infection Models with Swine Cells.
Pathogens
June 2023
Swine and Poultry Infectious Diseases Research Center (CRIPA) and Groupe de Recherche sur les Maladies Infectieuses en Production Animale, Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte St., Saint-Hyacinthe, QC J2S 2M2, Canada.
Bacterial and/or viral co-infections are very common in swine production and cause severe economic losses. , and are pathogenic bacteria that may be found simultaneously in the respiratory tracts of pigs. In the present study, the interactions of with epithelial and phagocytic cells in the presence or absence of a pre-infection with and/or were studied.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!