Objectives: This pilot study aimed to investigate the feasibility of non-invasively assessing synovial tissue hypoxia in vivo using photoacoustic (PA) imaging in a post-traumatic osteoarthritis model and explore its correlation with OA severity.
Methods: The three-dimensional vasculature structure and oxygenation level of synovial tissues of destabilization of the medial meniscus (DMM)-induced osteoarthritis (OA) mice were longitudinally monitored using PA imaging. Vascular volume/tissue volume (%) and tissue oxygen saturation (sO) were validated against results obtained by established Power Doppler (PD) imaging and dynamic changes of inhaled O concentration respectively. PA changes were correlated with the histological grading of cartilage damages.
Results: PA-measurements of vascularity and sO demonstrated a strong correlation with localized blood flow detected by PD imaging (r = 0.506, p < 0.001) and inhaled O concentration. DMM knees exhibited much more vascularity in synovial tissue at 4 months after surgery (median 11.3%, IQR: 10.7-15.5%) than the intact knees at time zero (median:5.1%, IQR:3.8-6.8%, p < 0.001) as well as the sham-operated knees (median: 4%, IQR: 3.75-5.45%, p = 0.017). Paradoxically, synovial tissue sO was significantly lower in DDM knees (median: 37.7%, IQR: 36.4-40.6%) than both the intact (47.1%, IQR: 41.9-49.8% p = 0.001) and sham-operated knees (45.1% IQR: 45.1-52.4%, p = 0.017). The PA-detected synovial tissue hypoxia correlated with the severity of cartilage loss in DMM mice (rho = -0.597, p = 0.031).
Conclusion: Here, we demonstrated PA imaging can be implemented for non-invasive imaging of the synovial tissue. Under PA imaging, synovitis in OA was characterized by increased angiogenesis and synovial tissue hypoxia; the latter was associated with the severity of OA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.pbiomolbio.2018.03.009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Near-infrared-II-driven Z-scheme heterojunction Polyglycolated MoS/CoFeO amplified edge potential for dual-mode imaging guided tumor synergistic therapy.
J Colloid Interface Sci
December 2024
School of Basic Medicine, General Hospital of Ningxia Medical University, Ningxia Medical University, Yinchuan 750004, China. Electronic address:
Targeting the peculiarities of tumor tissue microenvironment different from normal tissue, such as lower pH and overexpression of hydrogen peroxide is the key to effective treatment. In this study, acid-responsive Z-scheme heterojunctions polyglycolated MoS/CoFeO (MoS = molybdenum disulfide, CoFeO = cobalt ferrite) was synthesized using a two-step hydrothermal method, designated as MSCO-PEG, guided by dual modes of photoacoustic imagine (PAI) and nuclear magnetic imaging (MRI). MSCO-PEG (PEG = polyethylene glycol) responded to the acidic environment of tumor tissues and overexpression of hydrogen peroxide to turn on multimodal synergistic treatment of tumor cells under near-infrared-II (NIR-II) illumination.
View Article and Find Full Text PDFA stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imaging.
Nat Commun
January 2025
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
The shortwave infrared (SWIR) region is an ideal spectral window for next-generation bioimaging to harness improved penetration and reduced phototoxicity. SWIR spectral activity may also be accessed via supramolecular dye aggregation. Unfortunately, development of dye aggregation remains challenging.
View Article and Find Full Text PDFA mitochondria-targeted NIR fluorescence/photoacoustic dual-modality probe for highly sensitive and selective imaging of HClO in vivo.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
Department of Research & Clinical Laboratory, The Fifth Affiliated Hospital of Guangxi Medical University, The First People's Hospital of Nanning, Nanning 530022, Guangxi, PR China. Electronic address:
Article Synopsis
- Hypochlorous acid (HClO) is a reactive oxygen species produced in mitochondria, and its high levels are associated with various diseases, making its detection crucial in medicine.
- A new dual-modality probe called MB-ClO has been developed, which uses near-infrared fluorescence (NIRF) and photoacoustic (PA) imaging techniques to accurately and sensitively detect HClO in biological systems.
- MB-ClO shows low toxicity and good water solubility, has a fast response time, and has been successfully tested in a mouse model of rheumatoid arthritis, highlighting its potential for biomedical applications.
Detecting metastatic potential of cancer through longitudinal vasculature imaging of biomaterial scaffold using non-invasive photoacoustic microscopy and optical coherence tomography.
Theranostics
January 2025
Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Metastasis represents a stage in which the therapeutic objective changes from curing disease to prolonging survival, as detection typically occurs at advanced stages. Technologies for the early identification of disease would enable treatment at a lower disease burden and heterogeneity. Herein, we investigate the vascular dynamics within a synthetic metastatic niche as a potential marker of disease progression.
View Article and Find Full Text PDFCorrelation of Preclinical Imaging Modalities and Immunohistochemistry for Tumor Hypoxia and Vasculature.
In Vivo
December 2024
School of Physics, Mathematics and Computing, The University of Western Australia, Perth, Australia.
Background/aim: Tumors exhibit impaired blood flow and hypoxic areas, which can reduce the effectiveness of treatments. Characterizing these tumor features can inform treatment decisions, including the use of vasculature modulation therapies. Imaging provides insight into these characteristics, with techniques varying between clinical and preclinical settings.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!