We propose and demonstrate a new method which employs time-of-flight detection of femtosecond laser pulses for precise height measurement of large steps. By using time-of-flight detection with fiber-loop optical-microwave phase detectors, precise measurement of large step height is realized. The proposed method shows uncertainties of 15 nm and 6.5 nm at sampling periods of 40 ms and 800 ms, respectively. This method employs only one free-running femtosecond mode-locked laser and requires no scanning of laser repetition rate, making it easier to operate. Precise measurements of 6 μm and 0.5 mm step heights have been demonstrated, which show good functionality of this method for measurement of step heights.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1364/OL.43.001447 | DOI Listing |
J Allergy Clin Immunol
March 2025
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minn. Electronic address:
Background: The few reported patients with pathogenic IRF8 variants have manifested 2 distinct phenotypes: (1) an autosomal recessive severe immunodeficiency with significant neutrophilia and absence of or significant decrease in monocytes and dendritic cells and (2) a dominant-negative form with only a decrease in conventional type 2 dendritic cells (cDC2s) and susceptibility to mycobacterial disease.
Objectives: Genetic testing of a child with persistent EBV viremia identified a novel IRF8 variant: c.1279dupT (p.
Front Microbiol
February 2025
Department of Physiology, Faculty of Medicine, University of Tabuk, Tabuk, Saudi Arabia.
Antimicrobial resistance (AMR) is recognized as one of the foremost global health challenges, complicating the treatment of infectious diseases and contributing to increased morbidity and mortality rates. Traditionally, microbiological culture and susceptibility testing methods, such as disk diffusion and minimum inhibitory concentration (MIC) assays, have been employed to identify AMR bacteria. However, these conventional techniques are often labor intensive and time consuming and lack the requisite sensitivity for the early detection of resistance.
View Article and Find Full Text PDFJ Am Soc Mass Spectrom
March 2025
Department of Chemistry, University of California, Berkeley, California 94720-1460, United States.
Protein aggregation is a factor in a multitude of neurodegenerative diseases and aggregates of protein-based biotherapeutics can cause toxicity and adverse patient outcomes. Monoclonal antibody (M)-fluorophore (F) complexes with four different antibody sequences and masses of ∼680 kDa were analyzed using size-exclusion chromatography (SEC) and mass spectrometry using both quadrupole-time-of-flight (QTOF) and charge detection mass spectrometry (CDMS). Higher-order aggregates were not resolved using SEC, but species as large as the MF complex and MF were resolved using QTOF and CDMS, respectively.
View Article and Find Full Text PDFSurg Radiol Anat
March 2025
Department of Neuroendovascular Therapy, Saitama Sekishinkai Hospital, 2-37-20 Irumagawa, Sayama, Saitama, 350-1305, Japan.
Purpose: To describe a case of abnormal fusion between the long P1 segment of the posterior cerebral artery (PCA) and the long posterior communicating artery (PCoA) diagnosed by magnetic resonance angiography (MRA).
Methods: An 83-year-old woman with left pulsatile tinnitus underwent cranial MRA using a 3-Tesla scanner. MRA was performed using a standard 3-dimensional time-of-flight technique.
Front Fungal Biol
February 2025
Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
The emerging fungal pathogen () has been increasingly detected in environmental and animal samples, although studies in this regard are still scarce, especially in fisheries contexts. This study reports the first-time detection of in a commercially relevant fish species belonging to the Sciaenidae family ( sp.), indicating its potential emergence as a pathogen in Brazil.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!