Maduramicin is one of the most extensively used anticoccidial drugs for the treatment of Eimeria spp. infections. However, overdosage, misuse and drug interactions have resulted in the development of ionophore toxic syndrome. Heart and skeletal muscles have been identified as the main target organs of toxicity. In the present study, primary chicken myocardial cells were isolated to investigate the toxicity and underlying mechanisms of maduramicin. Our results showed that maduramicin causes morphological changes and a decrease in the viability of chicken myocardial cells. Annexin V-FITC/PI and 4',6-diamidino-2-phenylindole (DAPI) staining showed a significant increase in the number of apoptotic cells. Furthermore, caspases-3/8/9 were activated at the gene and protein levels and this was accompanied by the upregulation of apoptosis-related genes, including bcl-2, bax, and cytochrome C. Treatment with the pan-caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp (O-Me) fluoromethyl ketone (z-VAD-fmk) ameliorated the apoptosis and cytotoxicity. Furthermore, intracellular Ca and reactive oxygen species (ROS) were elevated, whereas mitochondrial membrane potential (MMP) and intracellular glutathione (GSH) decreased with exposure to maduramicin. The antioxidant N-acetyl-cysteine (NAC) had no significant effect on maduramicin-induced cytotoxicity and apoptosis. Taken together, our findings demonstrate that maduramicin is cytotoxic to primary chicken myocardial cells via caspase dependent and independent apoptotic pathways.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.tiv.2018.03.008 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mef2c- and Nkx2-5-Divergent Transcriptional Regulation of Chick WT1_76127 and Mouse Gm14014 lncRNAs and Their Implication in Epicardial Cell Migration.
Int J Mol Sci
November 2024
Cardiovascular Development Group, Department of Experimental Biology, University of Jaen, 23071 Jaen, Spain.
Cardiac development is a complex developmental process. The early cardiac straight tube is composed of an external myocardial layer and an internal endocardial lining. Soon after rightward looping, the embryonic heart becomes externally covered by a new epithelial lining, the embryonic epicardium.
View Article and Find Full Text PDFWild-type transthyretin cardiac amyloidosis and aortic stenosis: Can carpal tunnel syndrome help distinguish the chicken from the egg?
J Intern Med
November 2024
Referral Center for Cardiac Amyloidosis, Mondor Amyloidosis Network, GRC Amyloid Research Institute and Cardiology Department, APHP Henri Mondor Hospital, Créteil, France.
Article Synopsis
- - The study investigates the relationship between transthyretin wild-type cardiac amyloidosis (TTRwt-CA) and aortic stenosis (AS), proposing a bidirectional connection where each condition may influence the other and lead to systemic manifestations, including carpal tunnel syndrome (CTS).
- - In a sample of 411 TTRwt-CA patients, approximately 70% had CTS, which correlated with younger age, more severe cardiac remodeling, and a higher frequency of extracardiac symptoms, while AS occurred in 21% of those with CTS and 31% without.
- - The research indicates that CTS may differentiate two phenotypes of TTRwt-CA: one associated with systemic involvement and poorly calcified low-flow
25-Hydroxycholecalciferol Improves Cardiac Metabolic Adaption, Mitochondrial Biogenetics, and Redox Status to Ameliorate Pathological Remodeling and Functional Failure in Obese Chickens.
Antioxidants (Basel)
November 2024
Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan.
Broiler breeder hens allowed ad libitum (Ad) feed intake developed obesity and cardiac pathogenesis and thereby were susceptible to sudden death. A supplement of 69 µg 25-hydroxycholecalciferol (25-OH-D3)/kg feed rescued the livability of feed-restricted (R) and Ad-hens (mortality; 6.7% vs.
View Article and Find Full Text PDFHSP90 Enhances Mitophagy to Improve the Resistance of Car-Diomyocytes to Heat Stress in Wenchang Chickens.
Int J Mol Sci
October 2024
Key Laboratory of Tropical Animal Breeding and Epidemic Disease Research of Hainan Province, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570100, China.
Heat shock protein 90 (HSP90) is recognized for its protective effects against heat stress damage; however, the specific functions and underlying molecular mechanisms of HSP90 in heat-stressed cardiomyocytes remain largely unexplored, particularly in tropical species. In our study, Wenchang chickens (WCCs) were classified into two groups: the heat stress survival (HSS) group and the heat stress death (HSD) group, based on their survival following exposure to heat stress. Heat stress resulted in significant cardiomyocyte damage, mitochondrial dysfunction, and apoptosis in the HSD group, while the damage was less pronounced in the HSS group.
View Article and Find Full Text PDFOmega-3 fatty acids mitigate histological changes and modulate the expression of ACACA, PFK1 and ET-1 genes in broiler chickens under environmental stress: a pulmonary artery, cardiomyocyte and liver study.
Poult Sci
December 2024
Department of Animal and Poultry Science, Faculty of Agricultural Technology, University of Tehran, Pakdasht, Tehran, Iran.
The aim of this study was to investigate the effects of omega-3 fatty acids on blood biochemical parameters, histological changes in pulmonary artery, cardiomyocytes, and liver, as well as the expression of ACACA, PFK1, and ET-1 genes in broiler chickens under environmental stress (high stoking density). A total of 420 one-day-old male Ross broilers were used in a 2 × 2 factorial arrangements, with 2 levels of environmental stress (without and with stress; 9 and 17 birds/m, respectively) and 2 levels of omega-3 fatty acids (low and high; 0.057 and 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!