AI Article Synopsis
- Researchers created a new metabolic pathway to produce a synthetic version of trichothecene using enzymes from the fungus Fusarium sporotrichioides.
- They achieved this by providing a specific compound, 7-hydroxyisotrichodermin, to a modified strain of the fungus that lacked a crucial enzyme, resulting in the production of 7-hydroxy T-2 toxin.
- The resulting 7-hydroxy T-2 toxin showed significantly lower toxicity (10 times less) compared to the original T-2 toxin when tested on HL-60 cells, suggesting potential safety benefits.
Article Abstract
An artificial metabolic route to an unnatural trichothecene was designed by taking advantage of the broad substrate specificities of the T-2 toxin biosynthetic enzymes of Fusarium sporotrichioides. By feeding 7-hydroxyisotrichodermin, a shunt pathway metabolite of F. graminearum, to a trichodiene synthase-deficient mutant of F. sporotrichioides, 7-hydroxy T-2 toxin (1) was obtained as the final metabolite. Such an approach may have future applications in the metabolic engineering of a variety of fungal secondary metabolites. The toxicity of 7-hydroxy T-2 toxin was 10 times lower than that of T-2 toxin in HL-60 cells.
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| http://dx.doi.org/10.1021/acs.jnatprod.7b00398 | DOI Listing |
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