The serious cytotoxicity of tyramine attracted marked attention as it induced necrosis of human intestinal cells. This paper presented a novel and facile high performance thin-layer chromatography (HPTLC) method tailored for screening tyramine in cheese. Separation was performed on glass backed silica gel plates, using methanol/ethyl acetate/ammonia (6/4/1 v/v/v) as the mobile phase. Special efforts were focused on optimizing conditions (substrate preparation, laser wavelength, salt types and concentrations) of surface enhanced Raman spectroscopy (SERS) measurements directly on plates after derivatization, which enabled molecule-specific identification of targeted bands. In parallel, fluorescent densitometry (FLD) scanning at 380
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| http://dx.doi.org/10.1016/j.jfda.2017.07.007 | DOI Listing |
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Article Synopsis
- A new fluorometric method for detecting tyramine is introduced, utilizing the direct reaction between Au(III) and tyramine in a phosphate buffer to create fluorescent gold nanoclusters.
- After mixing Au(III) and buffer solutions and adding tyramine, a quick and stable fluorescence signal is generated, with analysis supported by STEM and Atomic Fluorescence Microscopy.
- This method accurately quantifies tyramine concentrations from 6.0x10 M to 1.2x10 M, demonstrating reliability and minimal interference from substances like putrescine and cadaverine, with successful application in testing tuna and cheese samples.
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