Natural Transformation in Vibrio parahaemolyticus: a Rapid Method To Create Genetic Deletions.

The Gram-negative bacterium is an opportunistic human pathogen and the leading cause of seafood-borne acute gastroenteritis worldwide. Recently, this bacterium was implicated as the etiologic agent of a severe shrimp disease with consequent devastating outcomes to shrimp farming. In both cases, acquisition of genetic material via horizontal transfer provided with new virulence tools to cause disease. Dissecting the molecular mechanisms of pathogenesis often requires manipulating its genome. Classically, genetic deletions in are performed using a laborious, lengthy, multistep process. Here, we describe a fast and efficient method to edit this bacterium's genome based on natural competence. Although this method is similar to one previously described, requires counterselection for curing of acquired plasmids due to its recalcitrant nature of retaining extrachromosomal DNA. We believe this approach will be of use to the community. Spreading of vibrios throughout the world correlates with increased global temperatures. As they spread, they find new niches in which to survive, proliferate, and invade. Therefore, genetic manipulation of vibrios is of the utmost importance for studying these species. Here, we have delineated and validated a rapid method to create genetic deletions in This study provides insightful methodology for studies with other species.