AI Article Synopsis
- A novel segetane and jatrophane diterpene were isolated from the methanol extract of a plant, along with five known diterpenoids with different skeletons.
- The structural analysis of these compounds was conducted using various sophisticated techniques like HRESIMS and several types of NMR experiments to determine their chemical structures.
- In tests on mouse lymphoma cells, the ingenane diterpenes exhibited cytotoxic effects, while segetane and jatrophane did not, although both segetane and one ingenane showed promising potential in reversing multidrug resistance.
Article Abstract
A novel segetane (1: ) and jatrophane diterpene (2: ), together with five known diterpenoids possessing segetane (3: ), jatrophane (4: ), and ingenane skeletons (5 - 7: ), were isolated from the methanol extract of All. The structure elucidation of the compounds was performed by means of extensive spectroscopic analysis, including HRESIMS and 1D (H, -modulated spin-echo carbon experiment) and 2D (HSQC, HMBC, COSY, NOESY) NMR experiments. The multidrug resistance reversing and cytotoxic effects of five diterpenes (1, 4: - 7: ) were studied on the L5178 mouse lymphoma cell line using rhodamine 123 accumulation and the MTT cell viability assay. Segetane and jatrophane diterpenes had no cytotoxic activity on the sensitive parent and multidrug resistance cells, while ingenane diterpenes showed a cytotoxic effect on both cell lines. Ingenanes 6: and 7: and segetane 1: demonstrated the remarkable multidrug resistance modulating effect at 20 µM.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1055/a-0589-0525 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!