Plasmonic nanoparticle-based expansion microscopy with surface-enhanced Raman and dark-field spectroscopic imaging.

Biomed Opt Express

Department of Electrical and Computer Engineering, University of Houston, 4800 Calhoun Rd., Houston, TX 77004, USA.

Published: February 2018

Fluorescence-based expansion microscopy (ExM) is a new technique which can yield nanoscale resolution of biological specimen on a conventional fluorescence microscope through physical sample expansion up to 20 times its original dimensions while preserving structural information. It however inherits known issues of fluorescence microscopy such as photostability and multiplexing capabilities, as well as an ExM-specific issue in signal intensity reduction due to a dilution effect after expansion. To address these issues, we propose using antigen-targeting plasmonic nanoparticle labels which can be imaged using surface-enhanced Raman scattering spectroscopy (SERS) and dark-field spectroscopy. We demonstrate that the nanoparticles enable multimodal imaging: bright-field, dark-field and SERS, with excellent photostability, contrast enhancement and brightness.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5854062PMC
http://dx.doi.org/10.1364/BOE.9.000603DOI Listing

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