Introduction: Resin sealers with biocompatible and bioactive additives have been used in clinical practice. Recently, a calcium silicate root canal sealer was introduced under the name BioRoot RCS (Septodont, Saint Maur-des-Fossés, France). The aim of this study was to evaluate the effects of BioRoot RCS on cell survival and proliferation of cultured cells in parallel with an epoxy resin sealer with calcium phosphate and calcium oxide and a salicylate resin sealer with mineral trioxide aggregate filler. The tested hypothesis was that BioRoot RCS is significantly less cytotoxic than the other tested sealers.
Methods: The experiments were performed on NIH/3T3 cells (American Type Culture Collection, Manassas, VA) grown as monolayer cultures at 37°C in atmosphere containing 5% CO in air and 100% relative humidity. The sealers' extracts (24 hours and 1 week) were applied to cells at 1:1 and 1:2 dilutions. The effect was assessed by a modified sulforhodamine B staining assay in reference to controls after 24 and 72 hours of exposure. All experiments were performed at least twice in 6 replicates. Analysis of variance and post hoc comparison tests were used to evaluate the statistical significance of the results at a level of significance of P = .05.
Results: BioRoot RCS was significantly less cytotoxic than the other 2 sealers. MTA-Fillapex (Angelus, Londrina, Brazil) and SimpliSeal (Discuss Dental, LLC, Calver City, CA) exhibited a similar antiproliferative profile with no statistically significant differences in all settings.
Conclusions: BioRoot RCS showed quite a positive biological behavior. Further investigation is needed in order to clarify the mechanism and the components that contribute to the beneficial results observed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.joen.2018.01.015 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assessment of a Light-Curable Hydrogel to Be Used for Root Canal Obturation.
J Dent Res
December 2024
Dentistry, School of Health Sciences, College of Medicine and Health, University of Birmingham, Birmingham, UK.
Root canal obturation involves filling of the chemomechanically prepared root canal space. Despite reduced microbial load, residual bacteria can still lead to reinfection and treatment failure. Currently, obturation techniques use a combination of gutta-percha and sealer, which requires the preparation of the root canal to specific sizes and tapers to enable the fitting of customized cones.
View Article and Find Full Text PDFA 4-year follow-up of root canal obturation using a calcium silicate-based sealer and a zinc oxide-eugenol sealer: A randomized clinical trial.
Int Endod J
February 2025
Department of Conservative Dentistry and Endodontics, University of Cagliari, Cagliari, Italy.
Aim: This randomized clinical trial assessed the outcomes of nonsurgical root canal treatment (RCT), comparing a calcium silicate-based sealer (CSBS) with the single-cone technique (SC) with a zinc oxide-eugenol (ZOE) sealer and warm vertical compaction (WVC).
Methodology: Ninety-two single- and multi-rooted teeth were divided into two groups and treated using either the SC with BioRoot™ RCS (BIO) or WVC with Pulp Canal Sealer™ EWT (PCS). Teeth with apical periodontitis (AP) in both groups were further divided into BIOAP and PCSAP subgroups.
A laboratory study to assess the physico-chemical properties of BioRoot RCS and BioRoot Flow exposed to citric acid and EDTA irrigating solutions.
Clin Oral Investig
November 2024
School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff, UK.
Objective: To assess physico-chemical properties of BioRoot RCS (powder-to-liquid formulation) and BioRoot Flow (pre-mixed formulation) after exposure to citric acid (CA) and EDTA.
Methods: BioRoot RCS and BioRoot Flow specimens (5 × 2 mm) were incubated for 28 days at 37 °C before being treated with test solution (distilled water, 17% EDTA, 10% CA, 20% CA or 40% CA). Changes in mass, ion release (inductively coupled plasma optical emission spectroscopy), phase composition (X-ray diffraction and Raman spectroscopy), surface morphology (scanning electron microscopy and energy dispersive spectroscopy) were evaluated.
Evaluation of the systemic effect of bone formation marker released by endodontic calcium silicate-based sealers in local tissues, the bloodstream, and body organs.
Odontology
September 2024
Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, USP, Bauru, São Paulo, Brazil.
Calcium silicate-based sealers are bioactive materials that release ions when in contact with body fluids. Therefore, this study aims mapping/trace bone formation markers released by MTA Fillapex, BioRoot RCS, and experimental tricalcium silicate-based sealer (CEO) into subcutaneous tissues, bloodstream and body organs. Toward, polyethylene tubes filled with sealers were implanted into connective tissue of Wistar rats.
View Article and Find Full Text PDFImpact of final rinse with saline or alcohol solution on root canal sealability.
Swiss Dent J
September 2024
University of Zurich, Clinic of Conservative and Preventive Dentistry, Center of Dental Medi-cine, 8032 Zurich, Switzerland.
Endodontic irrigation protocols are not only used to clean and disinfect the root canal system, but also to condition the canal wall dentine for subsequent root filling. In this study we tested whether a final irrigation step with saline solution or 80% ethanol improved root canal sealabilty by two popular sealers, an epoxy resin (AH Plus) and a hydraulic calcium silicate cement-based product (BioRoot RCS). Root canals in extracted single-rooted human teeth were instrumented and filled with a matched gutta-percha cone and sealer.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!