AI Article Synopsis

  • The study evaluated how progesterone (PG) affects ovarian ischemia-reperfusion (I/R) injury in female rats by measuring various biochemical and histopathological markers.
  • Rats were divided into three groups, including a control group, an I/R group, and an I/R plus PG group, with PG administered before surgery.
  • Results showed that rats treated with PG had lower total oxidant status levels and higher total antioxidant status compared to those who only experienced I/R injury, suggesting PG may offer protective effects against tissue damage.

Article Abstract

Objective: The aim of the present study was to evaluate the effects of progesterone (PG) against ovarian ischemia-reperfusion (I/R) injury through the evaluation of biochemical and histopathologic parameters.

Material And Methods: Twenty-one female Wistar albino rats were divided into three groups. Group 1: Sham; group 2: I/R; group 3: I/R+PG (8 mg/kg). PG was administered intraperitoneally to the rats in group 3, 30 minutes before a detorsion operation. Ovarian I/R injury was evaluated in serum and tissue by using biochemical parameters including malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index, neutrophil gelatinase-associated lipocalin (NGAL) and immunofluorescence staining by using a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay.

Results: Serum and tissue TOS levels were significantly lower in group 3 than in group 2. Tissue TAS levels were higher in group 3 than in group 2 (p<0.001). NGAL and MDA levels were similar between the groups. Histologic score, including vascular congestion, hemorrhage, polymorphonuclear neutrophils, and interstitial edema, was higher in group 2. Pre-treatment with PG decreased the score, but this difference was not statistically significant. The number of apoptotic cells was higher in group 2 than in groups 1 and 3. The TUNEL-positive cell number decreased with PG in group 3.

Conclusion: Preoperative PG treatment might exert protective effects on ovarian I/R injury through its anti-apoptotic and antioxidative properties.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994815PMC
http://dx.doi.org/10.4274/jtgga.2017.0047DOI Listing

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