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Corrigendum to "Surface display and characterization of recombinant α-l-Rhamnosidase from Emiliania huxleyi on Pichia pastoris" [Bioorg. Chem. 155 (2025) 108121].
Bioorg Chem
January 2025
Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Surface display and characterization of recombinant α-l-Rhamnosidase from Emiliania huxleyi on Pichia pastoris.
Bioorg Chem
February 2025
Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
An α-l-Rhamnosidase gene with an open reading frame of 3192 bp encoding a 1036-amino acid protein (EhRha) was cloned from Emiliania huxleyi for flavonoid hydrolysis on the cell surface of Pichia pastoris (P. pastoris) strain GS115 by fusing with the anchor protein (AGα1) from Saccharomyces cerevisiae. Fluorescence microscopy and flow cytometry assays revealed that EhRha was successfully displayed on the cell surface of P.
View Article and Find Full Text PDFExploring proteins within the coccolith matrix.
Sci Rep
December 2024
Department of Earth Sciences, University of Oxford, South Parks Rd, Oxford, OX1 3AN, UK.
Coccolithophores comprise a major component of the oceanic carbon cycle. These unicellular algae produce ornate structures made of calcium carbonate, termed coccoliths, representing ~ 50% of calcite production in the open ocean. The exact molecular mechanisms which direct and control coccolith formation are unknown.
View Article and Find Full Text PDFStability of a surrogate African swine fever-like algal virus in corn- and soybean-based feed ingredients during extended storage and digestion processes.
Front Vet Sci
November 2024
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States.
Prevention of transmission of African swine fever virus (ASFV) through contaminated feed ingredients and complete feed is an important component of biosecurity protocols for global feed supply chains. Use of extended storage times for feed ingredients has become a popular and emerging mitigation strategy that may allow partial inactivation of ASFV before manufacturing swine feeds. However, the effectiveness of this strategy is unclear because limited studies have been conducted using diverse methodologies and insufficiently sensitive measures of virus viability of only a few types of feed matrices.
View Article and Find Full Text PDFMicrozooplankton grazing on the coccolithophore and its role in the global calcium carbonate cycle.
Sci Adv
November 2024
Marine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA.
Identifying mechanisms driving the substantial dissolution of biogenic CaCO (60 to 80%) in surface and mesopelagic waters of the global ocean is critical for constraining the surface ocean's alkalinity and inorganic carbon budgets. We examine microzooplankton grazing on coccolithophores, photosynthetic calcifying algae responsible for a majority of open-ocean CaCO production, as a mechanism driving shallow dissolution. We show that microzooplankton grazing dissolves 92 ± 7% of ingested coccolith calcite, which may explain 50 to 100% of the observed CaCO dissolution in supersaturated surface waters.
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