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Development of a universal, tunable, miniature fluorescence microscope for use at the point of care. | LitMetric

Fluorescence microscopy can be a powerful tool for cell-based diagnostic assays; however, imaging can be time consuming and labor intensive to perform. Tunable systems give the ability to electronically focus at user selected depths inside an object volume and may simplify the opto-mechanical design of the imaging system. We present a prototype of a universal, tunable, miniature fluorescence microscope built from poly(methyl methacrylate) singlets that incorporates miniature, electrowetted lenses for electronic focusing. We demonstrate the ability of this system to perform clinically relevant differential white blood cell counts using single use custom cartridges pre-loaded with the fluorescent dye acridine orange.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846512PMC
http://dx.doi.org/10.1364/BOE.9.001041DOI Listing

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