We compared lactate threshold (T) with non-invasive markers of an aerobic-anaerobic transition; namely, ventilatory (VT) and tissue saturation index (TSI) thresholds. While identification of a breakpoint in blood lactate concentration ([BLa]) is common for determination of an aerobic-anaerobic transition, non-invasive measures, VT and NIRS, have also received attention as a means of determining this critical exercise intensity. We hypothesised that one or other of these non-invasive measures would have a strong association with T. Thirty-one (n=31) competitive male athletes (mean ± SD, age 29±9 yr, height 1.81±0.1 m, body mass 77.7±10.0 kg) performed graded incremental cycling to volitional exhaustion. Heart rate, TSI and gas exchange data were measured throughout and [BLa] was determined at fixed intervals. Threshold detection involved a segmented linear regression analysis minimising the squared sum of the residuals to determine T, TSI and VT. Workload and HR at T, VT and TSI were analysed using repeated measures ANOVA and correlation assessed using Pearson's and interclass correlation coefficients. Thresholds at TSI and T were not significantly different (255±35 vs. 249±30 W, >0.05), suggesting that limitations in O delivery could be closely linked to an aerobic-anaerobic transition. However, poor correlation (r=0.55, ICC=0.54 and 95%LoA of +67 and -54 W) suggested other factors may exert an influence. Mean VT occurred at a significantly higher workload than T (271 ±35 vs 249±30 W, <0.001). Consequently, VT proved less useful, giving an indication of when an aerobic-anaerobic transition had already occurred. In conclusion, non-invasive markers of the aerobic transition are not concurrent with T
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http://dx.doi.org/10.70252/BUCT5185 | DOI Listing |
Microorganisms
November 2024
Istituto per la Bioeconomia, Consiglio Nazionale delle Ricerche, Via Madonna del Piano 10, Sesto Fiorentino, 50019 Firenze, Italy.
Background: Dark anaerobiosis promotes the acidification of the thylakoid lumen and a reduction in the plastoquinone (PQ) pool. The relationship between the reduction in the PQ pool in the dark and the induction of the xanthophyll cycle under high light stress was investigated in .
Methods: To achieve a comprehensive oxidative/reductive (aerobic/anaerobic conditions) state of the PQ pool, cultures were bubbled with air or nitrogen for 4 h.
J Environ Manage
November 2024
School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, Hunan, China. Electronic address:
Inoculating sulfate-reducing bacteria (SRB) habitats offers an eco-friendly method for treating sulfate-metal laden wastewater, characterized by high sulfate levels, low pH, and elevated heavy metals. This study optimizes source habitat selection of SRB by evaluating groundwater, sewage sludge, and lake sediment, focusing on their suitability and adaptability to aerobic-anaerobic transitions in industrial settings. Sewage sludge, with its slightly acidic pH, reducing environment, and high nutrient levels (Total organic carbon: 207.
View Article and Find Full Text PDFJ Environ Manage
August 2024
College of Engineering, Northeast Agricultural University, Harbin, 150030, China.
This research aimed to design an integrated aerobic-anaerobic reactor with dynamic aeration that was automatically regulated based on real-time oxygen concentration and investigate the aerobic pretreatment and subsequent dry co-anaerobic digestion (co-AD) characteristics of highly solids-loaded corn stover and swine manure in terms of temperature rise, physiochemical characteristics, and methane production. The high-temperature feedstocks from the aerobic pretreatment phase rapidly entered the AD phase without transportation and effectively improved the start-up and methane production of the co-AD. Oxygen concentration range, aeration rate, and pretreatment time affected the cumulative aeration time, temperature rise, and organic matter removal interactively during aerobic pretreatment, and a low aeration rate was relatively preferable.
View Article and Find Full Text PDFOsteoarthritis Cartilage
July 2024
Department of Chemical Engineering, Toronto Metropolitan University, Toronto, Ontario, Canada; Li Ka Shing Knowledge Institute, St. Michael's Hospital, Toronto, Ontario, Canada; Institute of Biomedical Engineering, Science and Technology (iBEST), Unity Health and Toronto Metropolitan University, Toronto, Ontario, Canada. Electronic address:
Objective: Chondrocytes, which typically rely on anaerobic metabolism, exhibit upregulated biosynthetic activity when subjected to conditions that elicit mixed aerobic-anaerobic metabolism. Previously, we observed that increasing media volume resulted in the transition from anaerobic to mixed aerobic-anaerobic metabolism. Maximal extracellular matrix (ECM) accumulation occurred at this transition as a result of changes in hypoxia-inducible factor 1α signaling and associated hypoxic gene expression.
View Article and Find Full Text PDFEnviron Sci Technol
March 2024
Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan.
Microbial methane oxidation has a significant impact on the methane flux from marine gas hydrate areas. However, the environmental fate of methane remains poorly constrained. We quantified the relative contributions of aerobic and anaerobic methanotrophs to methane consumption in sediments of the gas hydrate-bearing Sakata Knoll, Japan, by geochemical and microbiological analyses coupled with C-tracer incubation experiments.
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