AI Article Synopsis
- Previous studies indicate that BAP1 levels provide important prognostic information for uveal melanomas, but research on fine needle aspiration biopsies and their correlation with prognosis was lacking.
- An experimental study was conducted with fine needle aspiration biopsies from 113 uveal melanoma patients to assess BAP1 expression alongside chromosome 3 analysis and gene expression profiling.
- The findings show that low BAP1 nuclear expression correlates strongly with monosomy of chromosome 3, suggesting that BAP1 immunocytochemistry could be a valuable prognostic tool for uveal melanoma when sufficient tissue is available.
Article Abstract
Purpose: Prior studies have shown that nuclear reactivity for BRCA1-associated protein-1 (BAP1) yields prognostic information for paraffin-embedded uveal melanomas. Lacking are immunocytochemical studies of BAP1 on fine needle aspiration biopsies of uveal melanoma that correlate with prognosis or other markers of prognosis. Our purpose was to fill this gap.
Design: Experimental laboratory study.
Methods: Fine needle aspiration biopsies were performed prospectively on 113 patients with uveal melanomas, garnering limited subsets of cases for comparison. Agreement between immunocytochemistry for BAP1 nuclear staining vs chromosome 3 ploidy analysis and gene expression profiling was assessed by 2 × 2 contingency table analysis.
Results: The presence or absence of suppression of nuclear expression of BAP1 was strongly associated (73%, P = .000002) with monosomy and disomy chromosome 3, respectively. BAP1 nuclear expression was also correlated with gene expression profiling. Chromosome 3 ploidy analysis correlated with gene expression profiles.
Conclusion: When adequate material is obtained, immunocytology using BAP1 is a potentially informative tool for prognostication of uveal melanoma.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915968 | PMC |
| http://dx.doi.org/10.1016/j.ajo.2018.03.005 | DOI Listing |
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