AI Article Synopsis
- Noninvasive methods to detect early pancreatic cancer and its spread could significantly improve patient outcomes, as this disease typically has a very poor prognosis.
- Current diagnostic techniques, like PET scans, struggle with pancreatic ductal adenocarcinoma (PDAC) due to a lack of identifiable targets.
- Researchers developed a RAGE-specific single chain antibody (scFv) for PET imaging, which showed strong potential in detecting PDAC in mouse models, indicating it could be promising for future clinical applications.
Article Abstract
Noninvasive detection of both early pancreatic neoplasia and metastases could enhance strategies to improve patient survival in this disease that is notorious for an extremely poor prognosis. There are almost no identifiable targets for non-invasive diagnosis by positron emission tomography (PET) for patients with pancreatic ductal adenocarcinoma (PDAC). Over-expression of the receptor for advanced glycation end products (RAGE) is found on the cell surface of both pre-neoplastic lesions and invasive PDAC. Here, a RAGE-specific single chain (scFv) was developed, specific for PET imaging in syngeneic mouse models of PDAC. An anti-RAGE scFv conjugated with a sulfo-Cy5 fluorescence molecule showed high affinity and selectivity for RAGE expressing pancreatic tumor cells and genetically engineered KRASG12D mouse models of PDAC. An in vivo biodistribution study was performed with the 64Cu-radiolabled scFv in a syngeneic murine pancreatic cancer model, demonstrating both the feasibility and potential of an anti-RAGE scFv for detection of PDAC. These studies hold great promise for translation into the clinic.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846720 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0192821 | PLOS |
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RAGE-specific single chain Fv for PET imaging of pancreatic cancer.
PLoS One
June 2018
Molecular Imaging Laboratory, Department of Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
Article Synopsis
- Noninvasive methods to detect early pancreatic cancer and its spread could significantly improve patient outcomes, as this disease typically has a very poor prognosis.
- Current diagnostic techniques, like PET scans, struggle with pancreatic ductal adenocarcinoma (PDAC) due to a lack of identifiable targets.
- Researchers developed a RAGE-specific single chain antibody (scFv) for PET imaging, which showed strong potential in detecting PDAC in mouse models, indicating it could be promising for future clinical applications.
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