Adventitious roots (AR) are de novo formed roots that emerge from any part of the plant or from callus in tissue culture, except root tissue. The plant tissue origin and the method by which they are induced determine the physiological properties of emerged ARs. Hence, a standard method encompassing all types of AR does not exist. Here we describe a method for the induction and analysis of AR that emerge from the etiolated hypocotyl of dicot plants. The hypocotyl is formed during embryogenesis and shows a determined developmental pattern which usually does not involve AR formation. However, the hypocotyl shows propensity to form de novo roots under specific circumstances such as removal of the root system, high humidity or flooding, or during de-etiolation. The hypocotyl AR emerge from a pericycle-like cell layer surrounding the vascular tissue of the central cylinder, which is reminiscent to the developmental program of lateral roots. Here we propose an easy protocol for in vitro hypocotyl AR induction from etiolated Arabidopsis seedlings.
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http://dx.doi.org/10.1007/978-1-4939-7747-5_7 | DOI Listing |
Plant Sci
January 2025
Anhui Province Key Laboratory of Forest Resources and Silviculture, School of Forestry and Landscape Architecture, AnHui Agricultural University, HeFei 230036, PR China. Electronic address:
Trichome development and anthocyanin accumulation are regulated by a complex regulatory network, the MBW complexes consist of MYB, bHLH, and WD40 transcription factors. In this study, two sequences, named PaTTG1.1, and PaTTG1.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Jiangxi Provincial Key Laboratory of Plant Germplasm Innovation and Genetic Improvement, Lushan Botanical Garden, Chinese Academy of Sciences, Jiujiang 332900, China.
Adventitious root (AR) formation in plants originates from non-root organs such as leaves and hypocotyls. Auxin signaling is essential for AR formation, but the roles of other phytohormones are less clear. In , at least two distinct mechanisms can produce ARs, either from hypocotyls as part of the general root architecture or from wounded organs during de novo root regeneration (DNRR).
View Article and Find Full Text PDFPlant Sci
December 2024
Department of Life Sciences, Presidency University, 86/1 College Street, Kolkata, WB 700073, India. Electronic address:
Ubiquitous to every stratum of life, cryptochromes regulate numerous light dependent functions in terrestrial plants. These include light-dependent transcription, circadian rhythm, inhibition of hypocotyl elongation, programmed cell death, promotion of floral initiation, mediation of gravitropic response, responding to biotic and abiotic stress etc. There have been quite a few seminal reviews including on plant cryptochromes, focusing mostly on the detailed functional aspects.
View Article and Find Full Text PDFBMC Plant Biol
December 2024
College of Agronomy, Sichuan Agricultural University, Chengdu, 611130, China.
Phytochrome-interacting factors (PIFs) belong to a subfamily of the bHLH transcription factor family and play a pivotal role in plant light signal transduction, hormone signal pathways, and the modulation of plant responses to various abiotic stresses. The soybean (Glycine max) is a significant food crop, providing essential oil and nutrients. Additionally, it is a vital industrial raw material and a lucrative cash crop.
View Article and Find Full Text PDFPhotochem Photobiol
December 2024
Graduate School of Informatics, Nagoya University, Nagoya, Japan.
Circadian clocks facilitate organisms' adaptation to the day-night environmental cycle. Some of the component genes of the clocks ("clock genes") respond directly to changes in ambient light, supposedly allowing the clocks to synchronize to and/or oscillate robustly in the environmental cycle. In the dicotyledonous model plant Arabidopsis thaliana, the clock genes CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), LATE ELONGATED HYPOCOTYL (LHY) and PSEUDO-RESPONSE REGULATOR 9 (PRR9) show transient expression in response to the morning light.
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