A two-photon fluorescent probe for Cu and S has been strategically prepared with naphthalimide derivative platform (NPE) covalently grafted onto the surface of magnetic core-shell FeO@SiO nanoparticles. The probe (NPE-FeO@SiO) exhibits selective response to Cu with enhanced fluorescence and efficient separation of Cu with external magnetic field. The consequent product NPE-FeO@SiO-Cu of NPE-FeO@SiO and Cu can work as an excellent sensor for S by removing Cu from the complex with fluorescence decreased, recovering the fluorescence of the probe. Therefore, the constituted Off-On-Off type fluorescence monitoring system means the probe is resumable. Moreover, the probe has been used to quantitatively detect Cu and S with low detection limits, which are 0.28 μM and 0.12 μM, respectively. Furthermore, the probe shows low cytotoxicity and excellent membrane permeability, which has been successfully applied for monitoring Cu and S in living cells and imaging Cu in deep-tissue with two-photon excited fluorescence.
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