A flow cytometric (FC) method was developed to retrieve particle size distributions (PSDs) and real refractive index (n) information in natural waters. Geometry and signal response of the sensors within the flow cytometer (CytoSense, CytoBuoy b.v., Netherlands) were characterized to form a scattering inversion model based on Mie theory. The procedure produced a mesh of diameter and n isolines where each particle is assigned the diameter and n values of the closest node, producing PSDs and particle real refractive index distributions. The method was validated using polystyrene bead standards of known diameter and polydisperse suspensions of oil with known n, and subsequently applied to natural samples collected across a broad range of UK shelf seas. FC PSDs were compared with independent PSDs produced from data of two LISST-100X instruments (type B and type C). PSD slopes and features were found to be consistent between the FC and the two LISST-100X instruments, but LISST concentrations were found in disagreement with FC concentrations and with each other. FC n values were found to agree with expected refractive index values of typical marine particle components across all samples considered. The determination of particle size and refractive index distributions enabled by the FC method has potential to facilitate identification of the contribution of individual subpopulations to the bulk inherent optical properties and biogeochemical properties of the particle population.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1364/AO.57.001705 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Transformative Gamified Binocular Therapy for Unilateral Amblyopia in Young Children: Pilot Prospective Efficacy and Safety Study.
JMIR Serious Games
January 2025
Department of Ophthalmology, Eye and ENT Hospital of Fudan University, No.83 Fenyang Road, Xuhui District, Shanghai, 200031, China, 86 021-64377134.
Background: Amblyopia is a common cause of visual impairment in children. Compliance with traditional treatments for amblyopia is challenging due to negative psychosocial impacts. Recent shifts in amblyopia treatment have moved from suppressing the dominant eye to enhancing binocular visual function.
View Article and Find Full Text PDFOptical diffraction tomography using a self-reference module.
Biomed Opt Express
January 2025
Department of Electronic Engineering, Maynooth University, Maynooth, Co. Kildare, Ireland.
Optical diffraction tomography enables label-free, 3D refractive index (RI) imaging of biological samples. We present a novel, cost-effective approach to ODT that employs a modular design incorporating a self-reference holographic capture module. This two-part system consists of an illumination module and a capture module that can be seamlessly integrated with any life-science microscope using an automated alignment protocol.
View Article and Find Full Text PDFReal-World Evidence on Digital Therapeutics in Pediatric Amblyopia: Insights into Rapid Axial Elongation Risk.
Ophthalmology
January 2025
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Guangzhou, 510060, China. Electronic address:
Objective: To evaluate the impact of daily digital therapeutics (DTx) on the risk of rapid axial elongation (RAE) in children with amblyopia using real-world data.
Design: Retrospective cohort study.
Subjects: A total of 1,394 children aged 3 to 12 years with amblyopia, of which 477 received DTx.
Real-world outcomes on myopia management efficacy of diverse segmented defocus optics (DSDO) and defocus incorporated multiple segments (DIMS) spectacle lenses in Chinese children: An initial 12-month prospective clinical study.
J Optom
January 2025
Department of Ophthalmology, Peking University People's Hospital, Beijing, China; Eye Diseases and Optometry Institute, Beijing, China; Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases, Beijing, China; College of Optometry, Peking University Health Science Center, Beijing, China.
Purposes: To investigate the 12-month effectiveness of Diverse Segmented Defocus Optics (DSDO) and Defocus Incorporated Multiple Segments (DIMS) spectacle lenses in a real-world clinical population in myopic and pre-myopic Chinese children.
Methods: About 364 subjects prescribed DSDO or DIMS were enrolled. Axial length (AL) and cycloplegic spherical equivalent refraction (SER) changes over 12 months were measured.
Retrospective observational cohort study of premature neonates screened for ROP with 7 years of follow-up assessing the visual outcomes and adequacy of preschool visual screening.
BMJ Open Ophthalmol
December 2024
Ophthalmology, Royal Hospital for Children, Glasgow, UK.
Background: Very premature infants screened for retinopathy of prematurity (ROP) that do not develop ROP still experience serious visual developmental challenges, and while it is recommended that all children in the UK are offered preschool visual screening, we aimed to explore whether this vulnerable group requires dedicated follow-up.
Methods: We performed a real-world retrospective observational cohort study of children previously screened for ROP in NHS Greater Glasgow and Clyde (Scotland) between 2013 and 2015. We excluded those with any severity of ROP identified during screening.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!