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Development of a triplex crystal digital RT-PCR for the detection of PHEV, PRV, and CSFV.
Front Vet Sci
December 2024
College of Animal Science and Technology, Guangxi University, Nanning, China.
Porcine hemagglutinating encephalomyelitis virus (PHEV), porcine pseudorabies virus (PRV), and classical swine fever virus (CSFV) are currently prevalent worldwide and cause similar neurological symptoms in infected pigs. It is very important to establish a detection method that can rapidly and accurately detect and differentiate these three viruses. Targeting the PHEV N gene, PRV gB gene, and CSFV 5' untranslated region (5'UTR), three pairs of specific primers and probes were designed, and a triplex crystal digital reverse transcription-PCR (cdRT-PCR) was developed to detect PHEV, PRV, and CSFV.
View Article and Find Full Text PDFOptical sensors based on plasmonic nano-structures: A review.
Heliyon
December 2024
Faculty of Electrical and Computer Engineering, Semnan University, Semnan, Iran.
Optical sensors are among the most significant optical devices that have found extensive applications for THz sensing. Surface plasmon-based sensors have attracted increasing attention more than other kinds of optical sensors such as photonic crystal, optical fiber, and graphene sensors, owing to their compact footprint, fast reaction, and high sensitivity value. Therefore, this work reviews plasmonic sensor structures divided into three general categories.
View Article and Find Full Text PDFComprehensive microcrystal electron diffraction sample preparation for cryo-EM.
Nat Protoc
December 2024
Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, CA, USA.
Article Synopsis
- Microcrystal electron diffraction (MicroED) is an advanced structural method useful for analyzing a variety of samples, including small molecules and proteins, using cryogenic electron microscopy techniques.
- The method captures diffraction data through the continuous rotation of small 3D crystals while being observed by a high-speed camera, then utilizes X-ray crystallographic software for structure determination.
- This guide provides detailed protocols for preparing samples, emphasizing that individual crystals need tailored growth conditions, and aids those with backgrounds in biochemistry and crystallography in optimizing their MicroED experiments, which can take from one day to several weeks.
Background/objective: Little is known about the rates of rheumatic disease diagnosis among children during the COVID-19 pandemic. We examined the impact of the pandemic on the diagnosis of juvenile idiopathic arthritis (JIA) in the United States.
Methods: We performed a historical cohort study using US commercial insurance data (2016-2021) to identify children aged <18 years without prior JIA diagnosis or treatment in the prior ≥12 months.
Relationship Between Coronary Artery Calcium Score and Vulnerability of Culprit Plaque Assessed by OCT in Patients With Established Coronary Artery Disease.
Circ Cardiovasc Imaging
December 2024
Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston. (D.F., D.K., K.S., T.N., M.C., I.M., I.-K.J.).
Background: Coronary artery calcium score (CACS) is widely used for risk stratification. However, in patients with established coronary artery disease, its clinical implication and relationship with plaque vulnerability are unclear. We sought to correlate the CACS and plaque vulnerability assessed by optical coherence tomography.
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