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Colour formation by bacterial nitric oxide synthase in fermented sausages inoculated with and without Mammaliicoccus vitulinus.
Meat Sci
March 2025
China Light Industry Key Laboratory of Meat Microbial Control and Utilization, Hefei University of Technology, Hefei 230009, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address:
In the present study, a Bacillus subtilis expression system was used to overexpress the gene of nitric oxide synthase (NOS), and the NOS was subsequently purified and added to fermented sausages to assess its colouration ability. The results indicated that NOS activity in the nos-recombinant strain was approximately 58-fold higher than that in the wild-type strain (P < 0.05).
View Article and Find Full Text PDFA review of how colors clue us into gross diagnosis in domestic animals.
Vet Pathol
March 2025
Universidade Federal do Mato Grosso do Sul, Campo Grande, Brazil.
Different tissues have a normal color spectrum that reflects their cellular composition and/or metabolic features. Similarly, distinct color variations may occur in tissues that have undergone pathologic or nonpathologic changes. Common examples of color changes in domestic animal tissues include red (associated with erythrocytes, hemoglobin, and myoglobin), brown (ferric hemoglobin or myoglobin, suppurative inflammation, lipid oxidation, postmortem autolysis, formalin fixation, neoplasms arising from cytochrome-rich tissues), yellow (hemoglobin and iron degradation, biliary pigment and by-products, carotenes, keratin, necrosis, suppurative or fibrinous inflammation), green (hemoglobin and iron degradation, biliary pigment and by-products, meconium, eosinophilic or suppurative inflammation, oomycete and algal infections), white (lack of blood, adipose tissue and its neoplasms, chylous effusion, necrosis, mineralization, fibrosis, lymphoid tissue, round cell neoplasms), translucent (transudate, cysts), black to gray (hemoglobin and iron degradation, melanin, carbon, tattoos), and blue to purple (poorly oxygenated blood, tattoos).
View Article and Find Full Text PDFStudy of Minor Chromophores in Biological Tissues by Diffuse Optical Spectroscopy (Review).
Sovrem Tekhnologii Med
March 2025
PhD, Head of the Department of Radiophysical Methods in Medicine; Head of the Laboratory of Biophotonics; A.V. Gaponov-Grekhov Institute of Applied Physics of the Russian Academy of Sciences, 46 Ulyanov St., Nizhny Novgorod, 603950.
Diffuse optical spectroscopy (DOS) is a rapidly advancing non-invasive diagnostic technique to investigate biological tissue, based on probing the target object with optical radiation in the visible and/or near-infrared wavelength range and detecting the diffusely scattered light from the tissue. The signals obtained through DOS provide extensive information about the biochemical composition of tissues due to the presence of light-absorbing compounds known as chromophores. To date, DOS is widely employed to detect major chromophores such as deoxygenated (Hb) and oxygenated (HbO) hemoglobin, water, lipids, and melanin.
View Article and Find Full Text PDFMajor heme proteins hemoglobin and myoglobin with respect to their roles in oxidative stress - a brief review.
Front Chem
February 2025
Department of Biophysics, Molecular Biology and Bioinformatics, University College of Science, University of Calcutta, Kolkata, India.
Oxidative stress is considered as the root-cause of different pathological conditions. Transition metals, because of their redox-active states, are capable of free radical generation contributing oxidative stress. Hemoglobin and myoglobin are two major heme proteins, involved in oxygen transport and oxygen storage, respectively.
View Article and Find Full Text PDFThe aim of the study was to gain insight into the time course of whole-body electromyostimulation (WB-EMS)-induced inflammatory and endocrine factors over 72 h. Twelve healthy individuals (5 men, 7 women; 33.0 ± 12 years) randomly performed a single 20-minute high-intensity WB-EMS and a time- and intensity-matched whole-body circuit training (CT) with a 3-week wash-out period.
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