Femurs and tibias of normal rats were fixed in 4% unbuffered solutions of glutaraldehyde or formaldehyde containing 1% NaF. Following washing, radiographically controlled demineralization was performed in 3.25% HNO3. Light microscopy and transmission electron microscopy revealed the presence of so-called fluoride granules in bone trabeculae, cartilage, cartilage remnants, bone cells, chondrocytes and in the bone marrow. Selected area diffraction indicated that the fluoride granules contained CaF2. X-ray diffraction analysis of non-demineralized NaF-fixed bones were negative for CaF2. The results are discussed and related to precipitation artifacts reported in demineralized hard tissues and to similar CaF2-containing granules observed in demineralized bones and incisors of rats given high doses of NaF in diet or drinking water.
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| http://dx.doi.org/10.1111/j.1600-0722.1979.tb00697.x | DOI Listing |
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