AI Article Synopsis
- Mycobacterium tuberculosis is a major health threat, and researchers are studying two proteins, Rv0164 and MSMEG_0129, to understand their roles in the bacteria.
- Gene knockout studies reveal that MSMEG_0129 is crucial for bacterial growth, indicating that Rv0164 could be a potential target for new treatments.
- The crystal structure of MSMEG_0129 shows similarities to certain enzymes but suggests that these proteins may be involved in lipid transfer during cell envelope synthesis rather than catalyzing specific chemical reactions.
Article Abstract
Mycobacterium tuberculosis is a notorious pathogen that continues to threaten human health. Rv0164, an antigen of both T- and B cells conserved across mycobacteria, and MSMEG_0129, its close homolog in Mycobacterium smegmatis, are predicted members of the START domain superfamily, but their molecular function is unknown. Here, gene knockout studies demonstrate MSMEG_0129 is essential for bacterial growth, suggesting Rv0164 may be a potential drug target. The MSMEG_0129 crystal structure determined at 1.95 Å reveals a fold similar to that in polyketide aromatase/cyclases ZhuI and TcmN from Streptomyces sp. Structural comparisons and docking simulations, however, infer that MSMEG_0129 and Rv0164 are unlikely to catalyze polyketide aromatization/cyclization, but probably play an irreplaceable role during mycobacterial growth, for example, in lipid transfer during cell envelope synthesis.
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