The influence of arachidonic acid metabolites on PPAR and RXR expression in bovine uterine cells.

Gen Comp Endocrinol

Department of Biodiversity Protection, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Tuwima St. 10, 10-747 Olsztyn, Poland. Electronic address: http://tbr.pan.olsztyn.pl/images/KSIAZKA_KONFERENCYJNA_TBR_30_08_17.pdf.

Published: June 2018

AI Article Synopsis

  • PPARs are important transcription factors in the body that interact with ligands like metabolites from arachidonic acid, affecting gene expression related to various biological processes.
  • The study specifically examined how metabolites such as prostaglandins and leukotrienes influence the expression of different PPAR isoforms and their partners, RXRs, in bovine uterine cells.
  • Results showed that prostaglandins primarily impacted PPAR expression in stromal cells, while leukotrienes affected PPARγ in epithelial and myometrial cells; additionally, blocking PPAR signaling altered RXR expression, suggesting RXRs can compensate for lost PPAR function in certain cells.

Article Abstract

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the superfamily of nuclear receptors. Three isoforms have been described: alpha (PPARα), delta (PPARδ), and gamma (PPARγ). PPARs heterodimerize with retinoid X receptors (RXRs: RXRα, RXRβ and RXRγ). PPAR activity can be modulated by several ligands, including arachidonic acid (AA) metabolites. The aims of the study were to determine the effect of AA metabolites (prostaglandin [PG]E, PGF, leukotriene [LT]B, and LTC) on mRNA (real-time PCR) and protein expression (Western blotting) of PPARα, PPARδ, and PPARγ, and on mRNA expression of RXRα, RXRβ, and RXRγ, in bovine epithelial, stromal, and myometrial primary uterine cells and in bovine stromal cells with silenced PPAR genes (N = 10). All PPAR and RXR isoforms were expressed. Prostaglandins affected expression of PPARs only in stromal cells, whereas LTs modulated PPARγ mRNA expression in epithelial and myometrial primary cells. Blockade of signal transduction through PPARs prevented interactions between AA metabolites and PPARs and changed RXR expression comparing with primary stromal cells. In primary stromal uterine cells, mRNA expression of RXRs was higher than that of PPARs. In uterine stromal cells in which intracellular signaling through PPARs was blocked, RXRs seem to take over the role of PPARs and are pivotal for cell functions. This study revealed the reaction of PPARs and RXRs to agonists which naturally occur in the bovine uterus.

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http://dx.doi.org/10.1016/j.ygcen.2018.03.009DOI Listing

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