AI Article Synopsis
- A new labeling strategy using a short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb) allows for precise fluorophore labeling in fixed and living cells without disrupting biological targets.
- This method enables high-quality super-resolution imaging (dSTORM) of various proteins in both mammalian and yeast cells, ensuring minimal errors in labeling.
- The approach is versatile and could potentially make it easier to study a wider range of challenging cellular targets using advanced imaging techniques.
Article Abstract
Dense fluorophore labeling without compromising the biological target is crucial for genuine super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for fixed and living cells utilizing a short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb). BC2-tagging of ectopically introduced or endogenous proteins does not interfere with the examined structures and bivBC2-Nb staining results in a close-grained fluorophore labeling with minimal linkage errors. This allowed us to perform high-quality dSTORM imaging of various targets in mammalian and yeast cells. We expect that this versatile strategy will render many more demanding cellular targets amenable to dSTORM imaging.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834503 | PMC |
| http://dx.doi.org/10.1038/s41467-018-03191-2 | DOI Listing |
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