Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/NHH.0000000000000658 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evolution of the substrate specificity of an RNA ligase ribozyme from phosphorimidazole to triphosphate activation.
Proc Natl Acad Sci U S A
September 2024
Department of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114.
The acquisition of new RNA functions through evolutionary processes was essential for the diversification of RNA-based primordial biology and its subsequent transition to modern biology. However, the mechanisms by which RNAs access new functions remain unclear. Do RNA enzymes need completely new folds to support new but related functions, or is reoptimization of the active site sufficient? What are the roles of neutral and adaptive mutations in evolutionary innovation? Here, we address these questions experimentally by focusing on the evolution of substrate specificity in RNA-catalyzed RNA assembly.
View Article and Find Full Text PDFThe Effects of the 2020 BLM Protests on Racial Bias in the United States.
Pers Soc Psychol Bull
September 2024
Radboud University, Nijmegen, The Netherlands.
The 2020 Black Lives Matter (BLM) protests in response to the murder of George Floyd highlighted the lingering structural inequalities faced by Black people in the United States. In the present research, we investigated whether these protests led to reduced implicit and explicit racial bias among White U.S.
View Article and Find Full Text PDFPotential Biases in Test-Negative Design Studies of COVID-19 Vaccine Effectiveness Arising from the Inclusion of Asymptomatic Individuals.
Am J Epidemiol
August 2024
Faculty of Pharmacy, Université de Montréal, Montréal, Québec, Canada.
The test-negative design (TND) is a popular method for evaluating vaccine effectiveness (VE). A "classical" TND study includes symptomatic individuals tested for the disease targeted by the vaccine to estimate VE against symptomatic infection. However, recent applications of the TND have attempted to estimate VE against infection by including all tested individuals, regardless of their symptoms.
View Article and Find Full Text PDFA genetically encoded biosensor to monitor dynamic changes of c-di-GMP with high temporal resolution.
Nat Commun
May 2024
Biozentrum, University of Basel, Spitalstrasse 41, 4056, Basel, Switzerland.
Monitoring changes of signaling molecules and metabolites with high temporal resolution is key to understanding dynamic biological systems. Here, we use directed evolution to develop a genetically encoded ratiometric biosensor for c-di-GMP, a ubiquitous bacterial second messenger regulating important biological processes like motility, surface attachment, virulence and persistence. The resulting biosensor, cdGreen2, faithfully tracks c-di-GMP in single cells and with high temporal resolution over extended imaging times, making it possible to resolve regulatory networks driving bimodal developmental programs in different bacterial model organisms.
View Article and Find Full Text PDFEmerging Strategies for Modifying Cytochrome P450 Monooxygenases into Peroxizymes.
Acc Chem Res
January 2024
CAS Key Laboratory of Biofuels and Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
ConspectusCytochrome P450 monooxygenase is a versatile oxidizing enzyme with great potential in synthetic chemistry and biology. However, the dependence of its catalytic function on the nicotinamide cofactor NAD(P)H and redox partner proteins limits the practical catalytic application of P450 . An alternative to expensive cofactors is low-cost HO, which can be used directly to exploit the catalytic potential of P450s.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!