AI Article Synopsis
- Multidrug-resistant strains of Streptococcus pneumoniae were isolated from a patient in Sapporo, Japan, showing resistance to many antibiotics and only sensitivity to a few.
- The isolates shared the same serotype (19F) and genetic sequence (ST10017), indicating they likely come from a common source, with a previously identified strain showing different resistance patterns.
- Whole genome sequencing revealed several resistance mutations and genes, suggesting that the strain MDRSPN001 is evolving rapidly through genetic changes and acquiring resistance traits from other bacteria, including oral streptococci.
Article Abstract
Multidrug-resistant Streptococcus pneumoniae strains were isolated from blood and sputum of a patient with disseminated intravascular coagulation in Sapporo city, Japan. These antibiograms were only susceptible to vancomycin, linezolid, daptomycin, some carbapenems, and some fluoroquinolones. Identical antibiograms, serotypes (19F), and sequence types (ST10017) suggested a shared origin of these isolates. Only one ST10017 strain has been isolated in the same city in Japan previously (2014), and the 2014 isolate is still susceptible to macrolides. The whole genome of the blood-derived isolate was sequenced. The strain harbored resistance mutations in parC, gyrA, pbp1a, pbp2a, pbp2b, and pbp2x, and harbored the resistance genes, ermB and tetM. The nucleotide sequences of parC and pbp2x genes of strain MDRSPN001 were clearly different from those of other S. pneumoniae strains and were similar to those of oral streptococci strains. These findings suggest that strain MDRSPN001 has been rapidly and drastically evolving multidrug resistance by gene replacement and accumulation of genes originating from other strains, such as oral streptococci, Streptococcus mitis.
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| http://dx.doi.org/10.1016/j.jiac.2018.01.012 | DOI Listing |
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