AI Article Synopsis
- Advanced assays are needed to assess the immune potency of mesenchymal stem cells (MSCs) for clinical trials.
- Two assay systems tested—MSC secretome analysis and a quantitative RNA-based array—showed promise in measuring MSC potency by analyzing their immune-modulating properties.
- Results indicated a distinct cytokine profile from MSCs that correlates with T cell suppression, suggesting these methodologies could streamline and strengthen MSC potency analysis in research and clinical settings.
Article Abstract
Assays that can characterize MSC immune potency need to be identified for use in advanced clinical trials. MSCs possess a number of putative regenerative and immunomodulatory properties, and an assay matrix approach may best capture involved effector pathways. We have tested two assay systems to measure the potency of MSCs derived from human subjects: MSC secretome analysis and a quantitative RNA-based array for genes specific to immunomodulatory and homing properties of MSCs. Secretome analysis identified a unique cytokine signature that is upregulated by MSCs or downregulated in responder PBMCs and correlated with T cell suppression. Use of interferon-γ as a surrogate for the action of activated PBMCs on MSCs served as an alternative for the use of human PBMCs as responder cells in a potency assay. Our approach and results define and simplify the multifunctional or matrix responses of MSCs and may serve as a platform for robust potency analysis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855117 | PMC |
| http://dx.doi.org/10.1016/j.celrep.2018.02.013 | DOI Listing |
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