AI Article Synopsis
- CD137 is a molecule found on activated T cells that can help isolate T cells responding to specific viruses, as shown in mouse studies.
- Using a special CD137 monoclonal antibody created in their lab, researchers successfully isolated virus-specific CD8β T cells from the blood of pigs infected with different strains of PRRSV.
- The study found that these CD8β T cells expressed CD137 after stimulation and that the isolated virus-specific T cells were detected at low levels at specific days after infection, indicating the antibody's effectiveness for this application.
Article Abstract
CD137 is a costimulatory molecule transiently expressed on activated T cells after mitogen or antigen stimulation that can be exploited for isolating antigen-specific T cells as reported in mouse models. By utilizing an antiporcine CD137 monoclonal antibody (mAb, clone 3B9) developed in our laboratory, we isolated virus-specific CD8β T cells from peripheral blood of pigs experimentally infected with different porcine reproductive and respiratory syndrome virus (PRRSV) strains. Similar to mouse, porcine CD8β T cells also express CD137 transiently upon Concavalin A stimulation while the unstimulated cells did not. Most frequently, virus-specific CD8β T cells were isolated at low levels from peripheral blood of pigs experimentally infected with PRRSV strains VR2385, NADC20, and MN184B at 49 and 63 days postinfection. The results suggest that porcine CD137-specific mAb is a useful tool for isolating virus-specific CD8 T cells from peripheral blood and tissues of pigs after in vitro stimulation with viral antigen.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/vim.2017.0189 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring Peripheral and Cardiac Immune Responses in a Pneumosepsis Mouse Model with Group A Streptococcus.
Am J Respir Cell Mol Biol
January 2025
Rostock University Medical Center, Institute of Medical Microbiology, Virology and Hygiene, Rostock, Germany.
(Group A Streptococcus, GAS) is a human pathogen that causes local and systemic infections of the skin and mucous membranes. However, GAS is also found asymptomatically in the nasopharynx of infants. GAS infections, including pharyngitis and invasive pneumosepsis, pose significant public health concerns.
View Article and Find Full Text PDFChronic Myelomonocytic Leukemia with Central Nervous System Involvement in a Dog.
J Am Anim Hosp Assoc
January 2025
From Veterinary Neurological Center "La Fenice," Selargius, Italy (I.T., F.T., A.G.).
An 8 yr old, male, mixed-breed dog was presented with a 2 mo history of progressive weakness, worsened in the last 2 days before examination. Neurological examination revealed ambulatory tetraparesis, ataxia, and proprioceptive deficits in all four limbs. Menace response was reduced in the right eye and discomfort was detected on neck manipulation.
View Article and Find Full Text PDFMicrofilaria in the Urine of a Dog with Concurrent Urinary Bladder Sarcoma.
J Am Anim Hosp Assoc
January 2025
From the Department of Veterinary Clinical Services, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota (N.S., A.H., D.H.).
A 12 yr old female spayed American Staffordshire terrier was referred for stranguria, pollakiuria, and concern for a mass in the trigone of the urinary bladder. A urinalysis and abdominal ultrasound were performed to further investigate the bladder mass. Nematode larvae were identified in the urine, termed microfilaruria, while a caudal abdominal mass was found on ultrasound impinging on the urethra.
View Article and Find Full Text PDFStratification of Experimental LPS-Induced Systemic Inflammatory Response by Expression Level of Hif1a and NFkb Genes.
Bull Exp Biol Med
January 2025
Avtsyn Research Institute of Human Morphology, Petrovsky National Research Center of Surgery, Moscow, Russia.
It was previously found that the severity of LPS-induced systemic inflammatory response (SIRS) in rats is determined by resistance to hypoxia and the level of Hif1a expression. Individual differences in the level of Hif1a and NFkb expression in the liver were studied in relation to the severity of inflammatory and immune reactions in LPS-induced SIRS in rats without previous placement in a ventilated decompression chamber. During the early periods after SIRS modeling, rats with high expression of the Hif1a and NFkb genes associated with increased expression of pro- and anti-inflammatory cytokines are identified.
View Article and Find Full Text PDFDose effect of corticosteroids on peripheral lymphocyte profiles in patients with systemic lupus erythematosus.
Clin Rheumatol
January 2025
Department of Rheumatology and Immunology, Peking University People's Hospital, 11 Xizhimen South Street, Beijing, 100044, China.
Objective: To investigate the dose effect of methylprednisolone (MP) on peripheral lymphocyte profiles in patients with systemic lupus erythematosus (SLE). This study investigated the impact of varied MP doses on peripheral lymphocyte subtypes in SLE patients.
Methods: We conducted a prospective study involving 51 SLE patients, categorized into four groups (40 mg/day, 80 mg/day, 500 mg/day, and 1000 mg/day) based on the administered MP dosage during hospitalization.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!