Porcine intestinal lymphoid tissues synthesize estradiol.

J Vet Sci

Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois, Urbana-Campaign, IL 61802, USA.

Published: July 2018

Estradiol (17β-estradiol) is synthesized primarily in the gonads of both sexes and regulates the development and function of reproductive organs. Recently, we reported that intestinal lymphocyte homeostasis is regulated by estradiol synthesized in the endothelial cells of the high endothelial venules (HEVs) of mesenteric lymph nodes and Peyer's patches in mice. This observation prompted us to hypothesize that HEVs of intestinal lymphoid tissues are sites of estradiol synthesis across species. In this study, we examined whether estradiol is synthesized in the intestinal lymphoid tissues of adolescent piglets. Comparisons of estradiol levels in blood and tissue showed that estradiol concentrations in mesenteric lymph nodes and Peyer's patches were significantly higher than the level in serum. Reverse transcription polymerase chain reaction showed that porcine intestinal lymphoid tissues express mRNAs for steroidogenic enzymes (, , , and ), and immunohistochemical results in ilial tissue showed expression of aromatase (CYP19) in Peyer's patch-localized endothelial cells of HEVs. When mesenteric lymph node and Peyer's patch tissues were cultured , they produced estradiol. Taken together, the results indicate that mesenteric lymph nodes and Peyer's patches are sites of estradiol synthesis in adolescent piglets.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070586PMC
http://dx.doi.org/10.4142/jvs.2018.19.4.477DOI Listing

Publication Analysis

Top Keywords

intestinal lymphoid
16
lymphoid tissues
16
mesenteric lymph
16
lymph nodes
12
nodes peyer's
12
peyer's patches
12
estradiol
9
porcine intestinal
8
estradiol synthesized
8
endothelial cells
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!