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Spontaneously regressing mass-like soft tissue involvement in a child with chronic non-bacterial osteomyelitis: case-based review.
Rheumatol Int
January 2025
Department of Pediatric Rheumatology, Istanbul Medeniyet University, Istanbul, Turkey.
Chronic non-bacterial osteomyelitis (CNO) is an inflammatory bone disease, usually diagnosed in childhood. It is characterized by the presence of multifocal or unifocal osteolytic lesions that can cause bone pain and soft tissue swelling. CNO is known to have soft tissue involvement.
View Article and Find Full Text PDFDevelopment and application of a quadruple RT-qPCR assay for the simultaneous detection of NoV GI, NoV GII, and HAV in bivalve shellfish.
Appl Environ Microbiol
December 2024
School of Public Health, Hebei Medical University, Shijiazhuang, China.
To achieve rapid and simultaneous detection of NoV GI, NoV GII, and HAV, a quadruple real-time fluorescence quantitative PCR (RT-qPCR) assay was developed using MS2 bacteriophage as a process control virus. The quadruple RT-qPCR assay effectively detected NoV GI, NoV GII, HAV, and MS2 RNA with detection limits of 10 copies/μL, 10 copies/μL, 10 copies/μL, and 10 copies/μL, respectively, within 1 hour 50 minutes. The quadruple RT-qPCR assay could specifically detect NoV GI, NoV GII, HAV, and MS2 without cross-reactions with other common pathogens, demonstrating good reproducibility with intra-assay and inter-assay coefficients of variation all below 2.
View Article and Find Full Text PDFTopography of ischemic strokes in cancer-associated non-bacterial thrombotic endocarditis: A single-institution descriptive case series.
J Stroke Cerebrovasc Dis
December 2024
Department of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA. Electronic address:
Background: Very limited data indicate that patients with stroke from cancer-associated NBTE (Ca-NBTE) exhibit a characteristic pattern of widely distributed ischemic lesions of varying sizes.
Methods: An electronic search of Mayo Clinic records (03/31/2002-06/30/2022) with ensuing manual review of brain magnetic resonance imaging (MRI) was performed to identify topographic characteristics of stroke from Ca-NBTE.
Results: In 112 patients with Ca-NBTE, 92 (82.
Update on the diagnosis and treatment of CNO in children: a clinician's perspective.
Eur J Pediatr
November 2024
Division of Pediatric Rheumatology, Department of Pediatrics, University Hospitals Leuven, Leuven, Belgium.
Unlabelled: Chronic non-bacterial osteomyelitis (CNO) is caused by aseptic inflammation of bones, primarily driven by the innate immune system. CNO may display different clinical presentations (acute vs chronic, uni- vs multifocal) and is accompanied by other inflammatory disorders in up to a third of patients. Once considered a rare disorder, it has become clear that many patients were underdiagnosed.
View Article and Find Full Text PDFA host immune-related LncRNA and mRNA signature as a discriminant classifier for bacterial from non-bacterial sepsis in children.
Heliyon
November 2024
Department of Critical Care Medicine, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200062, China.
Article Synopsis
- The study investigates how non-coding RNA variations and immune responses differ in pediatric patients with bacterial versus non-bacterial sepsis, aiming to better understand their clinical features.
- Using advanced techniques like microarrays and weighted correlation network analysis, researchers identified key long non-coding RNAs (lncRNAs) and mRNAs that are associated with the immune response in these patients.
- A machine learning model was created to distinguish between bacterial and non-bacterial sepsis based on specific lncRNAs and mRNAs, showing high accuracy in both training and validation sets, and correlating well with inflammatory markers.
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