The essential oil of Thymus algeriensis was analyzed as a potential preservative in soft cheese. We developed a novel method to test the preserving properties of essential oil in soft cheese. Contamination incidence of Penicillium aurantiogriseum was absent after 30 d of storage at 4°C with 25 µL of essential oil added. The antimicrobial activity was tested against 8 bacteria and 8 fungi. Thymus algeriensis oil showed inhibitory activity against tested bacteria at 0.03 to 0.09 mg/mL, and bactericidal activity was achieved at 0.05 to 0.15 mg/mL. For antifungal activity, minimum inhibitory concentrations ranged between 0.01 and 0.04 mg/mL and minimum fungicidal concentrations between 0.01 and 0.04 mg/mL. Furthermore, the oil was also screened for antiradical activity using the 1,1-diphenyl-2-picrylhydrazyl assay. The results showed that the oil was active and achieved half-maximal inhibitory activity at 0.132 mg/mL. We used gas chromatography, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry to investigate the volatile compounds from the oil. Carvacrol was identified as the main compound in the oil, represented by 80.9% of the total constituents, followed by p-cymene (7.7%).
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http://dx.doi.org/10.3168/jds.2017-13714 | DOI Listing |
Sci Rep
October 2024
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig, 44519, Egypt.
Heliyon
October 2024
Laboratory of Microbiology, Farhat Hached University Hospital, Sousse, Tunisia.
Due to the increasing resistance prevalence to the last line of antibiotics, such as colistin, and the rising threat of multi-drug resistant bacteria, it is crucial to find alternative therapeutic options. The current study focuses on evaluating antibacterial activities alone and in combination with colistin of essential oil (TA-EO) against colistin-resistant , , and co-harboring -1 gene. GC/MS was used to determine the chemical composition of TA-EO.
View Article and Find Full Text PDFMol Biol Rep
April 2024
Laboratory of Nanobiotechnology and Valorisation of Medicinal Phytoresources, National Institute of Applied Sciences and Technology, Carthage University, Tunis Cedex B.P. 676, Tunis, 1080, Tunisia.
Background: Thymus algeriensis Boiss. et Reut. is one of the most widespread North African species of the genus Thymus L.
View Article and Find Full Text PDFFoods
October 2022
Chemistry Department, University of Beira Interior, 6201-001 Covilhã, Portugal.
Members of the Lamiaceae family are considered chief sources of bioactive therapeutic agents. They are important ornamental, medicinal, and aromatic plants, many of which are used in traditional and modern medicine and in the food, cosmetic, and pharmaceutical industries. In North Africa, on the Mediterranean side, there is the following particularly interesting Lamiaceous species: Willd.
View Article and Find Full Text PDFCell Mol Biol (Noisy-le-grand)
March 2023
Laboratory of Analytical Biochemistry and Biotechnology (LABAB), Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, Algeria.
Klebsiella pneumoniae producing extended-spectrum β-lactamases (ESBL) continues to pose huge therapeutic challenges in the treatment of infections, primarily urinary infections, due to its multidrug resistance to antibiotics. Therefore, there is a need for research on this topic to investigate ways to reduce the spread of antibiotic resistance, identify novel therapeutic approaches to treat these infections and gain a better understanding of the mechanisms of resistance. In this context, this study aimed to analyze the chemical composition of essential oils (EOs) of Thymus algeriensis, Syzygium aromaticum, and Eucalyptus globulus and assess their activity against K.
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