AI Article Synopsis
- Bioluminescence is produced by living organisms, primarily in marine life, through Ca-regulated photoproteins, which play vital roles in defense, feeding, and breeding.
- The review covers key marine photoproteins like aequorin and obelin, highlighting their use in biomedical research for measuring calcium in cells and developing binding assays.
- Recombinant photoproteins offer advantages such as high sensitivity and low interference, making them promising tools for advanced analytical methods in both in vitro and in vivo studies.
Article Abstract
Bioluminescence is the light produced by a living organism and is commonly emitted by sea life with Ca-regulated photoproteins being the most responsible for bioluminescence emission. Marine coelenterates provide important functions involved in essential purposes such as defense, feeding, and breeding. In this review, the main characteristics of marine photoproteins including aequorin, clytin, obelin, berovin, pholasin and symplectin from different marine organisms will be discussed. We will focused on the recent use of recombinant photoproteins in different biomedical research fields including the measurement of Ca in different intracellular compartments of animal cells, as labels in the design and development of binding assays. This review will also outline how bioluminescent photoproteins have been used in a plethora of analytical methods including ultra-sensitive assays and in vivo imaging of cellular processes. Due to their unique properties including elective intracellular distribution, wide dynamic range, high signal-to-noise ratio and low Ca-buffering effect, recombinant photoproteins represent a promising future analytical tool in several in vitro and in vivo experiments.
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| http://dx.doi.org/10.1016/j.biopha.2018.02.065 | DOI Listing |
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