AI Article Synopsis
- Cerebellar neural stem cells (NSCs) need Hedgehog-Gli (Hh-Gli) signaling for maintenance and express Nanog for self-renewal.
- Researchers used next-generation sequencing to compare mRNA and microRNA levels in NSCs and differentiated NSCs, identifying Foxm1 as a key gene regulated by Gli and Nanog.
- Foxm1 was found to control microRNAs that are crucial for NSC self-renewal, and interfering with these miRNAs hindered the ability of NSCs to form neurospheres, revealing a new regulatory network.
Article Abstract
Cerebellar neural stem cells (NSCs) require Hedgehog-Gli (Hh-Gli) signalling for their maintenance and Nanog expression for their self-renewal. To identify novel molecular features of this regulatory pathway, we used next-generation sequencing technology to profile mRNA and microRNA expression in cerebellar NSCs, before and after induced differentiation (Diff-NSCs). Genes with higher transcript levels in NSCs (vs. Diff-NSCs) included Foxm1, which proved to be directly regulated by Gli and Nanog. Foxm1 in turn regulated several microRNAs that were overexpressed in NSCs: miR-130b, miR-301a, and members of the miR-15~16 and miR-17~92 clusters and whose knockdown significantly impaired the neurosphere formation ability. Our results reveal a novel Hh-Gli-Nanog-driven Foxm1-microRNA network that controls the self-renewal capacity of NSCs.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824884 | PMC |
| http://dx.doi.org/10.1038/s41598-018-21876-y | DOI Listing |
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