Advancement in high-throughput screening methods of novel therapeutic proteins for early stage research and development, specifically mAbs, have given mid-scale (milligram to gram scale) purification groups access to more of these molecules. The available purification technologies built to support mid-scale production was not efficient or versatile enough to keep up with this surge. To remedy this problem, we have designed and built a custom instrument using an ÄKTA Pure. This system enables parallel processing up to 5 samples and has the versatility to perform 1- to 3-step purification processes in a single queue. Furthermore, a unique purification scheme can be selected for each of the five samples in the queue. Overall processing time has reduced by 83% compared to manual, non-parallel load methods. Here, we describe our novel approach and demonstrate the flexibility, speed and efficiency of the instrument.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.chroma.2018.02.018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reductive Removal of Antimony from Wastewater by a UV/Sulfite Process: Targeted Recovery of Strategic Metalloid Antimony.
Environ Sci Technol
January 2025
Environmental Protection Research Institute, Sinopec (Beijing) Research Institute of Chemical Industry Co., Ltd., Beijing 100013, China.
The removal of antimony from wastewater using traditional methods such as adsorption and membrane filtration generates large amounts of antimony-containing hazardous wastes, posing significant environmental threats. This study proposed a new treatment strategy to reductively remove and recover antimony from wastewater using an advanced UV/sulfite reduction process in the form of valuable strategic metalloid antimony (Sb(0)), thus preventing hazardous waste generation. The results indicated that more than 99.
View Article and Find Full Text PDFMolecular Mechanisms of Humic Acid in Inhibiting Silica Scaling during Membrane Distillation.
Environ Sci Technol
January 2025
Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, P. R. China.
Membrane distillation (MD) efficiently desalinizes and treats high-salinity water as well as addresses the challenges in handling concentrated brines and wastewater. However, silica scaling impeded the effectiveness of MD for treating hypersaline water and wastewater. Herein, the effects of humic acid (HA) on silica scaling behavior during MD are systematically investigated.
View Article and Find Full Text PDFThe Role of Pyroptosis-Related Gene Signature and Immune Infiltration in Juvenile Dermatomyositis.
Clin Cosmet Investig Dermatol
January 2025
Department of Clinical Laboratory, Central Hospital of Dalian University of Technology, Dalian, 116033, People's Republic of China.
Objective: Juvenile dermatomyositis (JDM) is a complex autoimmune disease, and its pathogenesis remains poorly understood. Building upon previous research on the immunological and inflammatory aspects of JDM, this study aims to investigate the role of pyroptosis in the pathogenesis of JDM using a comprehensive bioinformatics approach.
Methods: Two microarray datasets (GSE3307 and GSE11971) were obtained from the Gene Expression Omnibus database, and a list of 62 pyroptosis-related genes was compiled.
Modeling virus filtration: Materials, applications, and mechanism.
iScience
January 2025
Ralph E. Martin Department of Chemical Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
While various methods are employed to ensure the virus safety of finished products, virus filtration (VF) stands out as the preferred method for virus removal and purification of a wide variety of products owing to its capability of separating product molecules with more than 90% recovery and no change in molecule characteristics. The modeling of the virus removal process for VF membranes is based on the principles of microfiltration (MF) and ultrafiltration (UF), but with modifications for the much narrower separation difference, which is less than 2-fold for the separation of product molecules and virus particles. In this review, we introduce the materials and application of VF highlighting the unique characteristics properties of VF membranes through the steps of invention and subsequent development.
View Article and Find Full Text PDFSoil polluted system shapes endophytic fungi communities associated with : a field experiment.
PeerJ
January 2025
Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, China.
With the expansion of the mining industry, environmental pollution from microelements (MP) and red mud (RM) has become a pressing issue. While bioremediation offers a cost-effective and sustainable solution, plant growth in these polluted environments remains difficult. is one of the few plants capable of surviving in RM-affected soils.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!