Isolation, characterization, and antibiotic sensitivity assessment of Gallibacterium anatis biovar haemolytica, from diseased Egyptian chicken flocks during the years 2013 and 2015. | LitMetric
Gallibacterium anatis biovar haemolytica constitutes a part of the normal microflora in the upper respiratory and genital tracts of healthy chickens, but it is also associated with different pathological conditions. In the current study, 102 commercial chicken flocks suffering from respiratory disease and/or drop in egg production were investigated for the presence of G. anatis during 2013 and 2015. These flocks comprised 8 breeder, 32 layer, and 62 broiler flocks. By culture method, 20 flocks were found positive: one isolate derived from broiler breeders, 6 isolates from layers, and 13 isolates from broilers. G. anatis biovar haemolytica was identified by phenotyping and PCR. Additionally, partial genome sequencing of 11 isolates (5 layer isolates of 2013 and 6 broiler isolates of 2015) based on 16S rRNA and 23S rRNA gene sequences was performed and revealed 96.5% to 100% genetic relatedness. Antibiotic sensitivity of these isolates revealed that the 2013 isolates were highly susceptible to florfenicol while the isolates of 2015 were highly susceptible to cefotaxime. Gallibacterium anatis biovar haemolytica is a newly introduced bacteria in Egypt causing salpingitis, peritonitis, drop in egg production, and/or respiratory signs.
Antimicrobial resistance is recognized worldwide as one of the greatest threats to human and animal health and the environment. To evaluate the resistance rate of biovar haemolytica, which contributes to bacteremia, oophoritis, ovarian follicle degeneration, salpingitis, decreased egg production, and increased mortality in hens, strains isolated from the reproductive tracts of layers were analyzed. The oviducts were taken from three hens from each of 10 flocks manifesting clinical signs related to laying.
Gallibacterium anatis and Enterococcus cecorum are bacteria linked to opportunistic infections in poultry and occasionally cause infections in immunocompromised individuals from contaminated food.
A 78-year-old Japanese man with acute cholangitis was found to have a coinfection of these bacteria, which were identified as highly virulent and multidrug-resistant strains through genome sequencing.
The case highlights that a common bile duct stone can pose a risk for infections by these pathogens even in healthy individuals, underscoring the need for monitoring these bacteria in animals and understanding their effects on human health.
Facultad de Estudios Superiores Iztacala, UNAM, Av. De Los Barrios 1, Los Reyes Iztacala, 54090, Tlalnepantla, Edo de México, Mexico. Electronic address:
Gallibacterium anatis is a member of the Pasteurellaceae family and is an opportunistic pathogen that causes gallibacteriosis in chickens. Stress plays a relevant role in promoting the development of pathogenicity in G. anatis.
Introduction: is an opportunistic bacteria inducing a range of clinical signs in poultry. strains show multidrug resistance to antibacterial substances. The purpose of this study was to examine the susceptibility of biovar isolates collected from the respiratory, reproduction and gastrointestinal tracts of chickens to different antibiotics from various classes.
Gallibacterium anatis biovar haemolytica is a bacterium that is frequently associated with infections of the reproductive tract and respiratory system in poultry. To assess the current prevalence and resistance profile of these bacteria in Poland, we collected and investigated 63 strains of Gallibacterium from diseased domestic poultry flocks including geese, laying hens, breeding hens and an ornamental hen. Detailed characterization of the isolates included the analysis of phenotypic antimicrobial resistance profiles and biofilm formation ability.
