[ pro - angiogenic activity of hydatid cysts from experimentally infected mice].

Objective: To preliminarily study the pro-angiogenic activity of hydatid cysts against human umbilical vein endothelial cells and the transcriptional level of potential pro-angiogenic factors.

Methods: The hydatid cysts and protoscolex derived from experimentally infected mice were collected and cultured , then the human umbilical vein endothelial cells were stimulated by the supernatant and cyst fluid respectively, and the angiogenesis was observed and analyzed through a microscope and the angiogenesis mode of the software NIH Image J. Meanwhile, the mouse homologous proteins of matrix metalloproteinase-9 (MMP-9) and high mobility group box B1 (HMGB1) were identified in genome through sequence alignment, and their transcriptional levels in the cyst wall and protoscolex were analyzed.

Results: The culture supernatant of hydatid cysts significantly promoted human umbilical vein endothelial cells into tubes ( = 73.03, < 0.001), the transcriptions of MMP-9 and HMGB1 were detected in the cyst wall and protoscolex, and the transcriptional level of MMP-9 was higher in protoscolex ( = -11.65, < 0.001), while the level of HMGB1 was higher in hydatid cysts ( = 6.43, = 0.003).

Conclusions: Some parasite-derived pro-angiogenic molecules may exist in the supernatant of hydatid cysts, while further researches are required into their exact mechanisms.