Objective: To investigate the status of infection in the population in Nanjing City so as to provide the evidence for the prevention and control of cryptosporidiosis.
Methods: A total of 100 fecal samples were collected from each of three districts (Liuhe, Qixia and Gaochun) and one hospital (Nanjing Zhongda Hospital) in 2015 and 2016 respectively. The fecal samples were detected for with microscopy (by using the gold amine phenol-modified acid-fast staining) and the positive samples were detected again for the molecular biology confirming by using the fluorescence quantitative PCR.
Results: During the two years, 581 cases of normal population who lived in the city were surveyed and no Cryptosporidium infection was found. Among 202 cases of outpatients with chronic diarrhea, there were 9 positive cases with the microscope scanning method (4.46%), and among the 9 cases, 7 cases showed obvious logarithmic amplification curves showing positive nucleic acid, but 2 cases without the obvious logarithmic amplification curves, and the nucleic acid positive rate was 3.47%.
Conclusions: infection is not found in the normal population of Nanjing City, but the infection is found in the chronic diarrhea patients. The results imply that we should strengthen the detection of in the chronic diarrhea patients, so as to provide the evidence for improving the diagnosis and treatment of cryptosporidiosis.
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