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[Culture and characterization of spontaneous ascites cells isolated from ]. | LitMetric

[Culture and characterization of spontaneous ascites cells isolated from ].

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi

Zoology Key Laboratory of Hunan Higher Education, Research Center on Conservation and Utilization of Biological Resources in Dongting Lake Area, Hunan University of Arts and Science, Changde 415000, China.

Published: April 2016

AI Article Synopsis

Article Abstract

Objective: To isolate and culture the spontaneous ascites cells from under artificial conditions, so as to investigate the molecular mechanism at the cell level.

Methods: The cells were isolated from spontaneous ascites of artificially bred for 90 d, and were cultured and observed under a microscope. The differences of ascites cells among normal, spontaneous ascites and schistosomiasis infected samples of were compared. The lesion of tissue was observed simultaneously.

Results: There were no obvious organ tissue lesions in with spontaneous ascites, and the number and types of cells in peritoneal fluid were irregular and significantly changed. With the extension of culture time, the colonies appeared and there were a large number of vacuole-like cells in the cultured medium and sequentially presenting proliferation, deformation, disintegration and the fiber-like changes and could be passaged 3-4 d only.

Conclusions: The cells from with spontaneous ascites are similar to its abdominal cavity cells after infection of .

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Source
http://dx.doi.org/10.16250/j.32.1374.2015195DOI Listing

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