[Changes of liver fibrosis-related miRNAs induced by soluble egg antigen of ].

Objective: To investigate the expression of miRNA associated with hepatic fibrosis induced by soluble egg antigen stimulation in mouse hepatocytes (AML12), so as to lay the foundation for clarifying the mechanism of schistosome infection leading to hepatic fibrosis.

Methods: The expressions of miR-122, miR-182, miR-23b, miR27b and KSRP in AML12 cells treated with SEA were measured by q-PCR. KSRP protein in cell lyses was measured by Western blotting. AML12 cells were transfected with miR-27b precursor or anti-miR-27b for 24 h, then q-PCR was adopted to determine KSRP mRNA, and KSRP protein was detected by Western blotting.

Results: The expressions of miR-182, miR-23b and miR27b were decreased and miR-122 was increased in AML12 cells following SEA treatment (all < 0.05). An increase of mRNA and protein of KSRP expression was also observed in AML12 cells after SEA stimulation (both < 0.05). In addition, KSRP mRNA expression was not changed significantly in AML12 cells transfected with anti-miR-27b or miR-27b precursor, and miR27b precursor reduced KSRP protein expression as compared with the control. In contrast, the expression of KSRP protein was increased in the anti-miR-27b group and decreased in the miR-27b precursor group.

Conclusions: After the stimulation of SEA, the expressions of a variety of liver fibrosis-related miRNAs and KSRP change in murine hepatocytes, including miR-27b. And miR-27b can regulate the expression of KSRP. These findings might lay a foundation for further study on the molecular mechanism of fibrosis induced by schistosome infection.