Role and underlying mechanism of in oxidative damage.

Spermatogenesis-associated gene 12 () functions as an inhibitor in spermatogenesis and tumorigenesis. Our previous study demonstrated that SPATA12 may be induced in tumor cells by ultraviolet (UV) C-mediated DNA damage, suggesting its importance in maintaining genomic integrity. In order to understand whether and how responds to oxidative damage, the present study established a cellular model of oxidative stress by detecting the effect of HO on cell viability and intracellular superoxide dismutase activity, and the levels of glutathione and malondialdehyde (MDA). Quantitative polymerase chain reaction results demonstrated that HO upregulated the expression of , and a dual luciferase reporter gene assay indicated that transcription factor activator protein-1 (AP-1) was involved in the response of to oxidative stress. Through the exogenous expression of , it was identified that decreased the level of reactive oxygen species and MDA, and also may reduce the degree of cellular oxidative damage and apoptosis induced by HO. In addition, resveratrol was demonstrated to increase the expression of by activating AP-1, and it may be used as a nontoxic activator of the gene. In conclusion, these results suggest that is upregulated by oxidative stress via AP-1, and that the exogenous expression of protects against HO-induced oxidative damage and apoptosis.