AI Article Synopsis
- Impotence can be a big problem for men with prostate cancer after they have surgery, and researchers found that certain nerve cells can heal themselves.
- In a study with rats, they looked at how these healing cells grow and how a specific complex, DAPK/CIP2A, plays a role in this process.
- The researchers think that targeting CIP2A might help treat nerve injuries better after prostate surgery, which could improve erectile function.
Article Abstract
Purpose: Impotence is one of the major complications occurring in prostate cancer patients after radical prostectomy (RP). Self-repair of the injured nerve has been observed in animal models and in patients after RP. However, the downstream signalling is not well documented. Here, we found that the DAPK/CIP2A complex is involved in GAS6/AXL-related Schwann cell proliferation.
Materials And Methods: The 3 groups were a sham group, a 14-day post-bilateral cavernous nerve injury (BCNI) group and a 28-day post-BCNI group. Erectile function was assessed and immunohistochemistry was performed. The rat Schwann cell RSC96 line was chosen for gene knockdown, cell viability, western blot, immunofluorescence and co-immunoprecipitation assays.
Results: The intracavernosal pressure was low on the 14 day after BCNI and partially increased by the 28 day. GAS6 and p-AXL expression gradually increased in the cavernous nerve after BCNI. RSC96 cells incubated with a GAS6 ligand showed increased levels of p-ERK1/2 and p-AKT. Moreover, DAPK and CIP2A.p-AXL and p-DAPK and CIP2A complexes were identified by both immunoblotting and co-immunoprecipitation.
Conclusion: The DAPK/CIP2A complex is involved in GAS6/AXL-related Schwann cell proliferation. CIP2A inhibits PP2A activity, which results in p-DAPK(S308) maintenance and promotes Schwann cell proliferation. CIP2A is a potential target for the treatment of nerve injury after RP.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5814221 | PMC |
| http://dx.doi.org/10.18632/oncotarget.23978 | DOI Listing |
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