All-in-One Theranostic Nanoplatform Based on Hollow MoS for Photothermally-maneuvered Oxygen Self-enriched Photodynamic Therapy.

Unlabelled: Photodynamic therapy (PDT) kills cancer cells by converting tumor-dissolved oxygen into reactive singlet oxygen (O) using a photosensitizer under laser irradiation. However, pre-existing hypoxia in tumors and oxygen consumption during PDT can result in an inadequate oxygen supply, which in turn hampers PDT efficacy. Herein, an O self-sufficient nanotheranostic platform based on hollow MoS nanoparticles (HMoS) with oxygen-saturated perfluorohexane (O@PFH) and surface-modified human serum albumin (HSA)/chloride aluminium phthalocyanine (AlPc) (O@PFH@HMoS-HSA/AlPc), has been designed for the imaging and oxygen self-enriched photodynamic therapy (Oxy-PDT) of cancer.

Methods: The anti-cancer activity and intracellular O generation performance of the nanoparticles were examined using 4T1 cells. We also evaluated the multimodal imaging capabilities and anti-tumor efficiency of the prepared nanoparticles in vivo using a 4T1 tumor-bearing nude mouse model.

Results: This nanoplatform could achieve the distinct fluorescence (FL)/photoacoustic (PA)/X-ray computed tomography (CT) triple-model imaging-guided photothermally-maneuvered Oxy-PDT. Interestingly, the fluorescence and Oxy-PDT properties of O@PFH@HMoS-HSA/AlPc were considerably quenched; however, photothermal activation by 670 nm laser irradiation induced a significant increase in temperature, which empowered the Oxy-PDT effect of the nanoparticles. In this study, O@PFH@HMoS-HSA/AlPc demonstrated a great potential to image and treat tumors both and , showing complete tumor-inhibition over 16 days after treatment in the 4T1 tumor model.

Conclusion: O@PFH@HMoS-HSA/AlPc is promising to be used as novel multifunctional theranostic nanoagent for triple-modal imaging as well as single wavelength NIR laser triggered PTT/Oxy-PDT synergistic therapy.