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Alginate Oligosaccharide-Induced Modification of the and Quorum-Sensing Systems in Pseudomonas aeruginosa. | LitMetric

AI Article Synopsis

Article Abstract

plays a major role in many chronic infections. Its ability to readily form biofilms contributes to its success as an opportunistic pathogen and its resistance/tolerance to antimicrobial/antibiotic therapy. A low-molecular-weight alginate oligomer (OligoG CF-5/20) derived from marine algae has previously been shown to impair motility in biofilms and disrupt pseudomonal biofilm assembly. As these bacterial phenotypes are regulated by quorum sensing (QS), we hypothesized that OligoG CF-5/20 may induce alterations in QS signaling in QS regulation was studied by using CV026 biosensor assays that showed a significant reduction in acyl homoserine lactone (AHL) production following OligoG CF-5/20 treatment (≥2%; < 0.05). This effect was confirmed by liquid chromatography-mass spectrometry analysis of C-AHL and 3-oxo-C-AHL production (≥2%; < 0.05). Moreover, quantitative PCR showed that reduced expression of both the and systems was induced following 24 h of treatment with OligoG CF-5/20 (≥0.2%; < 0.05). Circular dichroism spectroscopy indicated that these alterations were not due to steric interaction between the AHL and OligoG CF-5/20. Confocal laser scanning microscopy (CLSM) and COMSTAT image analysis demonstrated that OligoG CF-5/20-treated biofilms had a dose-dependent decrease in biomass that was associated with inhibition of extracellular DNA synthesis (≥0.5%; < 0.05). These changes correlated with alterations in the extracellular production of the pseudomonal virulence factors pyocyanin, rhamnolipids, elastase, and total protease ( < 0.05). The ability of OligoG CF-5/20 to modify QS signaling in PAO1 may influence critical downstream functions such as virulence factor production and biofilm formation.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923140PMC
http://dx.doi.org/10.1128/AAC.02318-17DOI Listing

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