Synthesis, characterization and efficacy of silver nanoparticles against Aedes albopictus larvae and pupae.

Silver nanoparticles have been studied in a wide range of medical and entomological research works due to their eco-friendly aspects. In our study salicylic acid (SA) and its derivative, 3,5-dinitrosalicylic acid (DNS), were used in a one-step synthesis of silver nanoparticles (AgNPs). First, UV-vis absorption spectroscopy was used to detect the formation of AgNPs. Second, the synthesized nanoparticles were characterized using scanning electron microscope, transmission electron microscope; energy-dispersive spectroscopy, X-ray diffraction analysis and Fourier transform infrared spectroscopy. I, II, III and IV Instar larvae and pupae of Ae. Albopictus were exposed to various concentrations of SA, DNS and synthesized AgNPs for 24h to evaluate the larvicidal and pupicidal effect. In larvicidal bioassay of SA, moderate mortality was observed at 180ppm against Ae. Albopictus with LC values of 86, 108, 135 and 141ppm for instar larvae I, II, III and IV, respectively. Synthesized AgNPs showed highest mortality rate at 12ppm and the LC values of SAAgNPs were 1.2ppm (I), 1.4ppm (II), 1.8ppm (III), 2.0ppm (IV) and 1.4ppm (pupae). Whereas LC values of DNSAgNPs were 1.2ppm (I), 1.5ppm (II), 1.8ppm (III) 2.3ppm (IV) and 1.4ppm (pupae). Moreover, the investigations toward the systemic effect of the tested substances on the fourth instar larvae of Ae. albopictus was evaluated and the levels of total proteins, esterases, acetylcholine esterase, and phosphatase enzymes were found to be significantly decreased as compared with the control. These results highlight that SA-AgNPs and DNS-AgNPs are potential tools to control larval populations of mosquito.