Pedobacter mongoliensis sp. nov., isolated from grassland soil.

A Gram-stain-negative, rod-shaped, motile by gliding and strictly aerobic bacterial strain, named 1-32, was isolated from soil of the Ordos grassland in Inner Mongolia, PR China. Strain 1-32 showed highest 16S rRNA gene sequence similarities to Pedobacter luteus N7d-4 (95.4 %), Pedobacter oryzae DSM 19973 (95.3 %), 'Pedobacter xinjiangensis' 12157 (95.2 %) and Pedobacter tournemirensis TF5-37.2-LB10 (95.1 %). Phylogenetic analyses clustered strain 1-32 with 'P. xinjiangensis' 12157 and P. tournemirensis TF5-37.2-LB10. The DNA G+C content was 43.4 mol%. Menaquinone 7 was the main respiratory quinone. The predominant fatty acids (>5 %) were iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and C16 : 1ω5c. The polar lipids of strain 1-32 comprised phosphatidylethanolamine, two unidentified polar lipids, one unidentified glycolipid and two unidentified phospholipids. Strain 1-32 could be distinguished from the other members of the genus Pedobacter based on its phylogenetic distance and physiological and biochemical characteristics such as being negative for the assimilation of rhamnose and the activity of α-glucosidase. Therefore, strain 1-32 represents a novel species of the genus Pedobacter, for which the name Pedobacter mongoliensis sp. nov. is proposed. The type strain is 1-32 (=KCTC 52859=CCTCC AB 2017084).