AI Article Synopsis
- Guinea pigs are often used in tuberculosis vaccine research, and body weight loss is commonly used as a humane endpoint in these studies.
- During a chronic study, researchers observed labored breathing in some infected guinea pigs, prompting the need for more consistent humane endpoints.
- The study developed a method combining body weight loss, labored breathing, and activity level to estimate the time for euthanasia, supported by histological severity scores of lung lesions.
Article Abstract
Guinea pigs are a commonly used model for tuberculosis vaccine research. Loss of body weight is the most frequently described humane endpoint for animals used in these studies. During a chronic study, we noted labored breathing in some tuberculosis-infected guinea pigs. To develop consistent humane endpoints for these guinea pigs, we performed an observational study using multiple clinical signs. A combination of body weight loss, labored breathing, and activity level during handling estimated the time to euthanasia within approximately 7 d. Histologic severity scores of lesions in the cranial or caudal lung lobe (or both) supported clinical endpoints. This study presents humane endpoints for the refinement of studies using guinea pigs in tuberculosis research.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824138 | PMC |
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