The diversity of haloarchaea associated with different dry salt lakes in northeastern Algeria was investigated together with their potential of hydrolytic enzyme production. A total of 68 aerobic halophilic archaea were isolated from saline sediments. Based on the 16S rRNA gene sequencing, the isolates were assigned to seven phylotypes within the class Halobacteria, namely Haloarcula, Halococcus, Haloferax, Halogeometricum, Haloterrigena, Natrialba, and Natrinema. The results showed that Haloferax group was found to be dominant in all samples (30 isolates) (44%) with high diversity, followed by Halococcus spp. (13%) (9 isolates). All phylotypes are extreme halophiles and thermotolerant with the ability to grow at temperatures up to 48 °C. In addition, the screening for extracellular halophilic enzymes showed that 89.7% of the isolates were able to produce at least two types of the screened enzymes. The strains producing esterase, gelatinase, inulinase, cellulase and protease activities were the most diverse functional group. These data showed an abundant and diverse haloarchaeal community, detected in Algerian wetland ecosystems, presenting a promising source of molecules with important biotechnological applications.
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http://dx.doi.org/10.1016/j.micres.2017.12.011 | DOI Listing |
Environ Microbiome
December 2024
GFZ German Research Centre for Geosciences, Section Geomicrobiology, Potsdam, Germany.
The Eger Rift subsurface is characterized by frequent seismic activity and consistently high CO concentrations, making it a unique deep biosphere ecosystem and a suitable site to study the interactions between volcanism, tectonics, and microbiological activity. Pulses of geogenic H during earthquakes may provide substrates for methanogenic and chemolithoautotrophic processes, but very little is currently known about the role of subsurface microorganisms and their cellular processes in this type of environment. To assess the impact of geologic activity on microbial life, we analyzed the geological, geochemical, and microbiological composition of rock and sediment samples from a 238 m deep drill core, running across six lithostratigraphic zones.
View Article and Find Full Text PDFHeliyon
December 2024
Research Laboratory of Environmental Sciences and Sustainable Development, LR18ES32, University of Sfax, Tunisia.
The annotated and predicted genomes of five archaeal strains (AS1, AS2, AS8, AS11 and AS19), isolated from Sfax solar saltern sediments (Tunisia) and affiliated with , were performed by RAST webserver (Rapid Annotation using Subsystem Technology) and NCBI prokaryotic genome annotation pipeline (PGAP). The results showed the ability of strains to use a reduced semi-phosphorylative Entner-Doudoroff pathway for glucose degradation and an Embden-Meyerhof one for gluconeogenesis. They could use glucose, fructose, glycerol, and acetate as sole source of carbon and energy.
View Article and Find Full Text PDFSyst Appl Microbiol
December 2024
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address:
Four novel halophilic archaeal strains CGA53, CG83, FCH27, and SEDH24 were isolated from a soda lake and two saline lakes in China, respectively. Strain CGA53 showed the highest 16S rRNA gene similarity (92.6%) to Salinilacihabitans rarus AD-4, and the other three strains were found to be related to Halalkalicoccus species with similarities of 97.
View Article and Find Full Text PDFBioresour Bioprocess
December 2024
Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea.
Haloarchaea represents a unique group of microorganisms that have adapted to thrive in high-salt environments. These microbes produce distinctive biomolecules, some of which exhibit extraordinary properties. One such biomolecule is bacterioruberin, a prominent red-pigmented C carotenoid commonly found in halophilic archaea, renowned for its antioxidant properties and potential as a functional resource.
View Article and Find Full Text PDFAnimals (Basel)
December 2024
Key Laboratory of Conservation and Utilization of Biological Resources in the Tarim Basin, Alar 843300, China.
Carotenoids from different sources have different structures and functions, and their dietary components benefit the health of various organisms. The effects of halophilic Archaea-derived C50 carotenoids on poultry egg quality and gut microbiota remain largely unexplored. In this study, we isolated a carotenoid-secreting strain of , TRM89021, from the Pamir Plateau.
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